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采用 Czerny-Turner 设计的组合光谱仪进行双波长激发气溶胶荧光光谱检测。

Dual-wavelength-excitation aerosol fluorescence spectra detection using combined spectrometer with Czerny-Turner design.

作者信息

Chen Siying, Jia Yiwen, Chen He, Yang Wenhui, Luo Yupeng, Li Zhongshi, Deng Yanbao, Tan Wangshu, Guo Pan, Zhang Yinchao, Guo Jianshu, Hu Lingfei, Lv Meng

机构信息

School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China.

School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Sep 5;277:121260. doi: 10.1016/j.saa.2022.121260. Epub 2022 Apr 14.

DOI:10.1016/j.saa.2022.121260
PMID:35447557
Abstract

We developed a dual-wavelength-excitation aerosol fluorescence spectra detection device prototype. In our system, the 263 nm and 355 nm lasers are used to sequentially excite the fluorescence of aerosol stream, which is located spatially and temporally by two crossed infrared lasers; a bifurcated fiber bundle is applied to receive the fluorescence spectra of 274-463 nm and 374-565 nm. Besides, with a 32-channel photomultiplier tube as detector, a self-developed combined spectrometer with Czerny-Turner design is employed to detect the two band spectra in a preset timing sequence. Experiments show that the system can detect the fluorescence spectra, after dual-wavelength-excitation, of three intrinsic fluorophore samples and three bioaerosol samples.

摘要

我们研制了一种双波长激发气溶胶荧光光谱检测装置原型。在我们的系统中,263纳米和355纳米的激光用于依次激发气溶胶流的荧光,气溶胶流由两个交叉的红外激光在空间和时间上定位;采用分叉光纤束接收274 - 463纳米和374 - 565纳米的荧光光谱。此外,以32通道光电倍增管作为探测器,采用自行研制的具有切尔尼 - 特纳设计的组合光谱仪按预设的定时序列检测两个波段光谱。实验表明,该系统能够检测三种内源性荧光团样本和三种生物气溶胶样本在双波长激发后的荧光光谱。

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