Naw Haung, Võ Tuấn Cường, Lê Hương Giang, Kang Jung-Mi, Mya Yi Yi, Myint Moe Kyaw, Kim Tong-Soo, Shin Ho-Joon, Na Byoung-Kuk
Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea.
Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea.
Insects. 2022 Mar 25;13(4):322. doi: 10.3390/insects13040322.
is an important mosquito vector transmitting diverse arboviral diseases in Myanmar. Pyrethroid insecticides have been widely used in Myanmar as the key mosquito control measure, but the efforts are constrained by increasing resistance. Knockdown resistance () mutations in the voltage-gated sodium channel (VGSC) are related to pyrethroid resistance in . We analyzed the patterns and distributions of the mutations in in the Mandalay area of Myanmar. The segment 6 regions of domains II and III of were separately amplified from individual genomic DNA via polymerase chain reaction. The amplified gene fragments were sequenced. High proportions of three major mutations, including S989P (54.8%), V1016G (73.6%), and F1534C (69.5%), were detected in the of from all studied areas. Other mutations, T1520I and F1534L, were also found. These mutations represent 11 distinct haplotypes of the population. The S989P/V1016G/F1534C was the most prevalent, followed by S989P/V1016V and V1016G/F1534C. A quadruple mutation, S989P/V1016G/T1520I/F1534C, was also identified. High frequencies of concurrent mutations were observed in of Myanmar , suggesting a high level of pyrethroid resistance in the population. These findings underscore the need for an effective vector control program in Myanmar.
是缅甸传播多种虫媒病毒疾病的重要蚊媒。拟除虫菊酯类杀虫剂作为关键的蚊虫控制措施在缅甸被广泛使用，但这些努力受到日益增加的抗药性的限制。电压门控钠通道（VGSC）中的击倒抗性（）突变与拟除虫菊酯抗性有关。我们分析了缅甸曼德勒地区拟除虫菊酯抗性突变的模式和分布。通过聚合酶链反应从单个基因组DNA中分别扩增出结构域II和III的第6节段区域。对扩增的基因片段进行测序。在所有研究地区的中检测到高比例的三种主要拟除虫菊酯抗性突变，包括S989P（54.8%）、V1016G（73.6%）和F1534C（69.5%）。还发现了其他拟除虫菊酯抗性突变，T1520I和F1534L。这些拟除虫菊酯抗性突变代表了该种群的11种不同单倍型。S989P/V1016G/F1534C最为普遍，其次是S989P/V1016V和V1016G/F1534C。还鉴定出一种四重突变，S989P/V1016G/T1520I/F1534C。在缅甸的中观察到高频率的同时存在的拟除虫菊酯抗性突变，表明该种群对拟除虫菊酯具有高水平抗性。这些发现强调了缅甸需要一个有效的病媒控制计划。
