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巴基斯坦拉合尔地区埃及伊蚊和白纹伊蚊的杀虫剂抗性及潜在靶标-位点和代谢机制。

Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan.

机构信息

Laboratório de Fisiologia E Controle de Artrópodes Vetores, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Rio de Janeiro, RJ, 21040-360, Brazil.

Community Medicine Department, Bacha Khan Medical College Mardan, Mardan, Pakistan.

出版信息

Sci Rep. 2021 Feb 25;11(1):4555. doi: 10.1038/s41598-021-83465-w.

DOI:10.1038/s41598-021-83465-w
PMID:33633183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7907206/
Abstract

Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alpha-cypermethrin, and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR, High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes in resistance, we carried out synergist bioassay with both species and then checked the expression of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species were susceptible to organophosphates and the insect growth regulator, however resistant to all pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450 overexpression.

摘要

抗杀虫剂的埃及伊蚊种群最近在巴基斯坦被报道，对其控制构成了威胁。我们旨在评估来自拉合尔的埃及伊蚊和白纹伊蚊种群对世界卫生组织推荐的杀虫剂的敏感性，并研究代谢和靶标抗性机制。为此，我们首先用幼虫杀虫剂噻嗪酮和吡丙醚，以及成蚊杀虫剂马拉硫磷、氯菊酯、溴氰菊酯、α-氯氰菊酯和乙氰菊酯进行了生物测定。我们通过 qPCR、高分辨率熔解（HRM）和测序寻找击倒抗性突变（kdr）。为了探讨解毒酶在抗性中的作用，我们对两种物种进行了增效生物测定，然后检查了 CYP9M6、CYP9J10、CYP9J28、CYP6BB2、CCAe3a 和 SAP2 基因在埃及伊蚊中的表达。这两个物种对有机磷和昆虫生长调节剂敏感，但对所有拟除虫菊酯都有抗性。我们报告了 kdr 单倍型 1520Ile + 1534Cys 和 T1520 + 1534Cys 在埃及伊蚊中高频出现，而白纹伊蚊仅表现出 L882M 改变。PBO 增加了埃及伊蚊对氯菊酯的敏感性，表明 P450 基因参与了抗性的形成，事实上，CYP928 高度表达。我们推测拉合尔市的登革热传播媒介对拟除虫菊酯具有抗性，可能是由于多种机制，如 kdr 突变和 P450 过表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/7be1c7a81c84/41598_2021_83465_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/a3512fbd6493/41598_2021_83465_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/90a8ea820d02/41598_2021_83465_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/e425e4c7f09f/41598_2021_83465_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/d237c2eaed9c/41598_2021_83465_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/b6f2aec3df1c/41598_2021_83465_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/7be1c7a81c84/41598_2021_83465_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/a3512fbd6493/41598_2021_83465_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/90a8ea820d02/41598_2021_83465_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/e425e4c7f09f/41598_2021_83465_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/d237c2eaed9c/41598_2021_83465_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/b6f2aec3df1c/41598_2021_83465_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f8/7907206/7be1c7a81c84/41598_2021_83465_Fig6_HTML.jpg

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本文引用的文献

[1]
Evidence for both sequential mutations and recombination in the evolution of kdr alleles in Aedes aegypti.

PLoS Negl Trop Dis. 2020-4-17

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Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation.

PLoS Negl Trop Dis. 2020-4-16

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