Zając Marcin, Kotyńska Joanna, Worobiczuk Mateusz, Breczko Joanna, Naumowicz Monika
Doctoral School of Exact and Natural Sciences, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, Poland.
Department of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, Poland.
Membranes (Basel). 2022 Mar 26;12(4):366. doi: 10.3390/membranes12040366.
In recent years, many scientists have studied the effects of polymer micro- and nanostructures on living organisms. As it turns out, plastic can be a component of the blood of livestock, eaten by humans around the globe. Thus, it seems important to investigate possible changes in the physicochemical parameters and morphology of the cell membranes of blood morphotic elements (red blood cells and platelets) under the influence of polymer particles. The article presents research in which cell membranes were exposed to plain polystyrene (PS) and amino-functionalized polystyrene (PS-NH) of two different sizes. The polymers were characterized by infrared spectroscopy and dynamic light-scattering methods. To analyze possible changes caused by polymer exposure in the structure of the membranes, their zeta potentials were measured using the electrophoretic light-scattering technique. The concentration of the polymers, as well as the exposure time, were also taken into the consideration during the research. Based on the obtained results, we concluded that 100 and 200 nm PS, as well as 100 nm PS-NH, internalize into the cells. On the contrary, 200 nm PS-NH particles attach to cell membranes. Our study clearly shows that particle size and surface chemistry determine the interaction with biological membranes.
近年来，许多科学家研究了聚合物微观和纳米结构对生物体的影响。事实证明，塑料可能是全球人类食用的牲畜血液的组成部分。因此，研究聚合物颗粒影响下血液形态形成元素（红细胞和血小板）细胞膜的物理化学参数和形态的可能变化似乎很重要。本文介绍了一项研究，其中细胞膜暴露于两种不同尺寸的普通聚苯乙烯（PS）和氨基官能化聚苯乙烯（PS-NH）中。通过红外光谱和动态光散射方法对聚合物进行了表征。为了分析聚合物暴露可能引起的膜结构变化，使用电泳光散射技术测量了它们的zeta电位。研究过程中还考虑了聚合物的浓度以及暴露时间。根据所得结果，我们得出结论，100和200 nm的PS以及100 nm的PS-NH会内化到细胞中。相反，200 nm的PS-NH颗粒附着在细胞膜上。我们的研究清楚地表明，颗粒大小和表面化学决定了与生物膜的相互作用。
