Balida Lex Aliko P, Regalado Julia Theresa A, Teodosio Jade Joshua R, Dizon Kathryn Ann H, Sun Zhe, Zhan Zhao Qi, Blancaflor Jenny Marie D, Sollesta Jan Vincent N, Villorente Zenith M, Saludes Jonel P, Dalisay Doralyn S
Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Iloilo City 5000, Philippines.
Shimadzu Asia Pacific (SAP), Singapore Science Park I, Singapore 118264, Singapore.
Metabolites. 2022 Mar 23;12(4):279. doi: 10.3390/metabo12040279.
L. (pigeon pea, locally known in the Philippines as kadios) seed is a functional food with health benefits that extend beyond their nutritional value. seeds contain highly diverse secondary metabolites with enriched beneficial properties, such as antibacterial, anticancer, and antioxidant activities. However, the antibacterial activities of secondary metabolites from Philippine-grown , against multidrug-resistant have not been thoroughly described. Here, we investigated the in vitro antibacterial properties of seed against multidrug-resistant ATCC BAA-44 (MDRSA) and three other strains ( ATCC 25923, ATCC 6538, and coagulase-negative ) and, subsequently, identified the antibiotic markers against strains using mass spectrometry. Secondary metabolites from seeds were extracted using acetone, methanol, or 95% ethanol. Antibacterial screening revealed antibiotic activity for the acetone extract. Bioassay-guided purification of the acetone extract afforded three semi-pure high-performance liquid chromatography (HPLC) fractions exhibiting 32-64 µg/mL minimum inhibitory concentration (MIC) against MDRSA. Chemical profiling of these fractions using liquid chromatography mass spectrometry (LCMS) identified six compounds that are antibacterial against MDRSA. High-resolution mass spectrometry (HRMS), MS/MS, and dereplication using Global Natural Products Social Molecular Networking (GNPS)™, and National Institute of Standards and Technology (NIST) Library identified the metabolites as rhein, formononetin, laccaic acid D, crotafuran E, ayamenin A, and biochanin A. These isoflavonoids, anthraquinones, and pterocarpanoids from seeds are potential bioactive compounds against , including the multidrug-resistant strains.
L.（木豆，在菲律宾当地称为卡迪奥斯）种子是一种功能性食品，其健康益处超出了营养价值。种子含有高度多样的次生代谢产物，具有丰富的有益特性，如抗菌、抗癌和抗氧化活性。然而，菲律宾种植的木豆次生代谢产物对多重耐药金黄色葡萄球菌的抗菌活性尚未得到充分描述。在此，我们研究了木豆种子对多重耐药金黄色葡萄球菌ATCC BAA - 44（MDRSA）和其他三种金黄色葡萄球菌菌株（金黄色葡萄球菌ATCC 25923、金黄色葡萄球菌ATCC 6538和凝固酶阴性金黄色葡萄球菌）的体外抗菌特性，随后使用质谱法鉴定了针对金黄色葡萄球菌菌株的抗生素标志物。木豆种子中的次生代谢产物用丙酮、甲醇或95%乙醇提取。抗菌筛选显示丙酮提取物具有抗菌活性。对丙酮提取物进行生物测定指导的纯化，得到三个半纯的高效液相色谱（HPLC）馏分，对MDRSA的最低抑菌浓度（MIC）为32 - 64 μg/mL。使用液相色谱质谱联用（LCMS）对这些馏分进行化学分析，鉴定出六种对MDRSA有抗菌作用的化合物。通过高分辨率质谱（HRMS）、串联质谱（MS/MS）以及使用全球天然产物社会分子网络（GNPS）™和美国国家标准与技术研究院（NIST）图书馆进行的去重复分析，将这些代谢产物鉴定为大黄酸、芒柄花素、紫胶酸D、巴豆呋喃E、阿亚门宁A和鹰嘴豆芽素A。这些来自木豆种子的异黄酮、蒽醌和紫檀素类化合物是针对金黄色葡萄球菌，包括多重耐药菌株的潜在生物活性化合物。
