Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Iloilo City 5000, Philippines.
Maridan Industries, Inc., Jaro, Iloilo City 5000, Philippines.
Molecules. 2021 Mar 17;26(6):1688. doi: 10.3390/molecules26061688.
Honey exhibits antibacterial and antioxidant activities that are ascribed to its diverse secondary metabolites. In the Philippines, the antibacterial and antioxidant activities, as well as the bioactive metabolite contents of the honey, have not been thoroughly described. In this report, we investigated the in vitro antibacterial and antioxidant activities of honey from and , identified the compound responsible for the antibacterial activity, and compared the observed bioactivities and metabolite profiles to that of Manuka honey, which is recognized for its antibacterial and antioxidant properties. The secondary metabolite contents of honey were extracted using a nonionic polymeric resin followed by antibacterial and antioxidant assays, and then spectroscopic analyses of the phenolic and flavonoid contents. Results showed that honey extracts produced by exhibits antibiotic activity against pathogens as well as high antioxidant activity, which are correlated to its high flavonoid and phenolic content as compared to honey produced by . The bioassay-guided fractionation paired with Liquid Chromatography Mass Spectrometry (LCMS) and tandem MS analyses found the presence of the flavonoid isorhamnetin (3-methylquercetin) in honey extract, which was demonstrated as one of the compounds with inhibitory activity against multidrug-resistant ATCC BAA-44. Our findings suggest that Philippine honey produced by is a potential nutraceutical that possesses antibiotic and antioxidant activities.
蜂蜜具有抗菌和抗氧化活性，这归因于其多样的次生代谢物。在菲律宾，蜂蜜的抗菌和抗氧化活性以及生物活性代谢物含量尚未得到充分描述。在本报告中，我们研究了 和 蜂蜜的体外抗菌和抗氧化活性，鉴定了具有抗菌活性的化合物，并将观察到的生物活性和代谢物图谱与具有抗菌和抗氧化特性的麦卢卡蜂蜜进行了比较。使用非离子聚合树脂提取蜂蜜中的次生代谢物，然后进行抗菌和抗氧化测定，以及酚类和类黄酮含量的光谱分析。结果表明， 产生的蜂蜜提取物对 病原体具有抗生素活性，同时具有高抗氧化活性，这与其类黄酮和酚类含量高于 产生的蜂蜜有关。生物测定指导的分级分离与液相色谱-质谱联用（LCMS）和串联质谱分析发现， 蜂蜜提取物中存在类黄酮异鼠李素（3-甲基槲皮素），它被证明是对多药耐药 ATCC BAA-44 具有抑制活性的化合物之一。我们的研究结果表明， 生产的菲律宾蜂蜜是一种具有抗生素和抗氧化活性的潜在营养保健品。
