Doillon C J, Hembry R M, Ehrlich H P, Burke J F
Am J Pathol. 1987 Jan;126(1):164-70.
During wound healing, it has been suggested, modified fibroblasts rich in actin filaments are responsible for wound contraction. With the use of specific fluorescent probe (NBD-phallacidin), the distribution of actin filaments are compared in normal dermis and in several wound contraction models, including open and burn wounds and full and thin-thickness skin autografts. Fibroblasts of normal dermis are slightly stained with NBD-phallacidin. Fibroblasts with actin filaments are increased in autografts, particularly at Days 15 and 21 after grafting, and are prominent in open and burn wounds. The wound contraction rate is not directly related to the presence of actin-staining fibroblasts. After stabilization of the contraction of open or burn wounds, fibroblasts rich in actin filaments remain. The superficial layer of full-thickness skin graft contains a similar actin distribution without concomitant contraction. It is concluded that the distribution of actin-rich fibroblasts corresponds morphologically to previous areas of necrosis or injury.
在伤口愈合过程中，有人提出富含肌动蛋白丝的改性成纤维细胞负责伤口收缩。使用特定的荧光探针（NBD-鬼笔环肽），比较正常真皮以及包括开放性伤口、烧伤伤口和全厚及薄厚皮自体移植在内的几种伤口收缩模型中肌动蛋白丝的分布。正常真皮的成纤维细胞用NBD-鬼笔环肽染色轻微。富含肌动蛋白丝的成纤维细胞在自体移植中增加，特别是在移植后第15天和第21天，并且在开放性伤口和烧伤伤口中很突出。伤口收缩率与肌动蛋白染色的成纤维细胞的存在没有直接关系。在开放性或烧伤伤口收缩稳定后，富含肌动蛋白丝的成纤维细胞仍然存在。全厚皮移植的表层含有类似的肌动蛋白分布，但没有伴随的收缩。结论是，富含肌动蛋白的成纤维细胞的分布在形态上与先前的坏死或损伤区域相对应。
