Guo Chong-Tao, Luo Xin-Cheng, Ying Sheng-Hua, Feng Ming-Guang
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
J Fungi (Basel). 2022 Mar 23;8(4):334. doi: 10.3390/jof8040334.
The fluffy genes - are well-known players in the upstream developmental activation pathway that activates the key gene of central developmental pathway (CDP) to initiate conidiation in . Here, we report insignificant roles of their orthologs in radial growth of under normal culture conditions and different stresses although and were involved in respective responses to heat shock and HO. Aerial conidiation level was lowered in the deletion mutants of and (15%) less than of and (30%), in which the key CDP genes and were repressed consistently during normal incubation. The CDP-controlled blastospore production in submerged cultures mimicking insect hemolymph was abolished in the mutant with and being sharply repressed and decreased by 55% in the mutant with only being downregulated. The fungal virulence against a model insect was attenuated in the absence of more than of irrespective of normal cuticle infection or cuticle-bypassing infection (intrahemocoel injection). These findings unravel more important role of than of , but null roles of , in 's insect-pathogenic lifecycle and a scenario distinctive from that in .
蓬松基因是上游发育激活途径中广为人知的参与者，该途径激活中央发育途径（CDP）的关键基因以启动分生孢子形成。在此，我们报告了它们的直系同源物在正常培养条件和不同胁迫下对[具体真菌名称]径向生长的作用不显著，尽管[具体基因1]和[具体基因2]分别参与了对热休克和HO的反应。在[具体基因1]和[具体基因2]的缺失突变体中，气生分生孢子形成水平降低（约15%），低于[具体基因3]和[具体基因4]的缺失突变体（约30%），在正常培养期间，关键的CDP基因[具体基因5]和[具体基因6]在这些突变体中持续受到抑制。在模拟昆虫血淋巴的深层培养中，CDP控制的芽生孢子产生在[具体基因1]突变体中被消除，[具体基因2]和[具体基因3]受到强烈抑制，而在仅[具体基因4]下调的[具体基因5]突变体中减少了55%。无论正常表皮感染还是绕过表皮感染（血腔注射），在没有[具体基因1]的情况下，真菌对模式昆虫的毒力比没有[具体基因2]时减弱得更多。这些发现揭示了在[具体真菌名称]的昆虫致病生命周期中，[具体基因1]比[具体基因2]发挥着更重要的作用，但[具体基因3]没有作用，这是一种与[另一种真菌]不同的情况。
