Zhou Jinyuan, Wang Siqin, Xia Yuxian, Peng Guoxiong
Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 401331, China.
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing 401331, China.
J Fungi (Basel). 2024 Jul 4;10(7):468. doi: 10.3390/jof10070468.
Entomopathogenic fungi are valuable sources of biological pesticides, with conidial yield and quality being pivotal factors determining their broad applications. AzaR, a fungus-specific zinc-cluster transcription factor, is known to regulate the biosynthesis of polyketone secondary metabolites in ; however, its role in pathogenic fungi remains unclear. This study investigated the role of in the growth, development, and environmental tolerance of . deletion slowed down conidial germination rate, caused reduction in conidial yield, lowered fungal tolerance to UV radiation, did not affect fungal heat-shock tolerance, and increased fungal sensitivity to the cell-wall-destructive agent calcofluor white. Furthermore, deletion transformed microcycle conidiation to normal conidiation on the microcycle conidiation medium. Transcription profile analysis demonstrated that could regulate transformation of the conidiation pattern by controlling the expression of genes related to cell division, mycelium growth and development, and cell wall integrity. Thus, this study identified a new gene related to fungal conidiation and environmental tolerance, enriching our understanding of the molecular mechanism of microcycle conidiation and providing theoretical support and genetic resources for the development of high-yielding strains.
昆虫病原真菌是生物农药的重要来源，分生孢子产量和质量是决定其广泛应用的关键因素。AzaR是一种真菌特异性锌簇转录因子，已知其可调节聚酮类次生代谢产物的生物合成；然而，其在致病真菌中的作用仍不清楚。本研究调查了AzaR在[具体真菌名称未给出]生长、发育和环境耐受性中的作用。AzaR缺失减缓了分生孢子萌发率，导致分生孢子产量降低，降低了真菌对紫外线辐射的耐受性，不影响真菌的热休克耐受性，并增加了真菌对细胞壁破坏剂荧光增白剂的敏感性。此外，在微循环产孢培养基上，AzaR缺失将微循环产孢转变为正常产孢。转录谱分析表明，AzaR可通过控制与细胞分裂、菌丝生长发育和细胞壁完整性相关基因的表达来调节产孢模式的转变。因此，本研究鉴定了一个与真菌产孢和环境耐受性相关的新基因，丰富了我们对微循环产孢分子机制的理解，并为高产菌株的开发提供了理论支持和遗传资源。
