Flores Ana R, Rêma Alexandra, Mesquita João R, Taulescu Marian, Seixas Fernanda, Gärtner Fátima, Amorim Irina
ICBAS-School of Medicine and Biomedical Sciences, University of Porto, 4050-313 Porto, Portugal.
Institute of Pathology and Molecular Immunology, University of Porto (IPATIMUP), 4200-465 Porto, Portugal.
Vet Sci. 2022 Apr 1;9(4):172. doi: 10.3390/vetsci9040172.
E-cadherin (E-cad) is a cell-adhesion molecule known for its tumor-invasion suppressor function. E-cad expression was examined immunohistochemically in a series of canine tissue samples, including normal gastric mucosa (NGM; n = 3), gastric carcinomas (GC; n = 33), adjacent non-neoplastic mucosa (NNM; n = 32), neoplastic emboli (n = 16) and metastatic lesions (n = 9). The relationship between E-cad expression and clinicopathological features were investigated. In NGM, epithelial cells showed strong latero-lateral membranous expression of E-cad, and this pattern was considered normal. The membranous staining was preserved in all specimens of NNM (100%), whereas abnormal E-cad expression was found in 87.9% of the GCs. A marked difference in E-cad expression was observed between normal and malignant tissues (p < 0.0002). Abnormal E-cad expression was significantly more frequent in poorly/undifferentiated carcinomas (96%) and diffuse (95%) and indeterminate carcinomas (100%) than in well-differentiated/intestinal ones (62.5%; p = 0.0115 and p = 0.0392, respectively). There was significant association between abnormal E-cad expression and the depth of invasion (p = 0.0117), and the presence neoplastic emboli (p = 0.0194). No statistically significant differences in E-cad expression were observed concerning tumor location, histological type according to WHO classification, and presence of metastatic lesions. Therefore, deregulation of E-cad expression may play a role in canine gastric carcinogenesis and in tumor progression; moreover, it might be a prognostic tool for canine gastric cancer.
E-钙黏蛋白（E-cad）是一种以其肿瘤侵袭抑制功能而闻名的细胞黏附分子。采用免疫组织化学方法检测了一系列犬组织样本中的E-cad表达情况，这些样本包括正常胃黏膜（NGM；n = 3）、胃癌（GC；n = 33）、相邻非肿瘤性黏膜（NNM；n = 32）、肿瘤栓子（n = 16）和转移灶（n = 9）。研究了E-cad表达与临床病理特征之间的关系。在NGM中，上皮细胞显示出E-cad在细胞膜侧面的强表达，这种模式被认为是正常的。NNM的所有标本（100%）均保留了膜染色，而87.9%的GC中发现了异常的E-cad表达。正常组织和恶性组织之间在E-cad表达上观察到显著差异（p < 0.0002）。与高分化/肠型癌（62.5%；p分别为0.0115和0.0392）相比，低分化/未分化癌（96%）、弥漫性癌（95%）和不确定型癌（100%）中异常E-cad表达明显更常见。异常E-cad表达与侵袭深度（p = 0.0117）和肿瘤栓子的存在（p = 0.0194）之间存在显著关联。在肿瘤位置、根据世界卫生组织分类的组织学类型以及转移灶的存在方面，未观察到E-cad表达的统计学显著差异。因此，E-cad表达失调可能在犬胃癌发生和肿瘤进展中起作用；此外，它可能是犬胃癌的一种预后工具。
