Oda R P, Whiteis C A, Schmid P G, Lund D D
Am J Physiol. 1987 Jan;252(1 Pt 2):H125-30. doi: 10.1152/ajpheart.1987.252.1.H125.
A liquid chromatographic assay was used to determine the choline and acetylcholine concentrations in the four chambers of rat hearts 2, 4, and 8 days after transplantation into an abdominal site. Corresponding measurements were made in the hearts of host rats. We found regional cardiac acetylcholine concentrations in controls follow the nonuniform pattern seen with choline acetyltransferase (CAT) activity, being highest in the atria (8-12 nmol/g) and lower in the ventricles (0.7-1.6 nmol/g). Following transplantation, acetylcholine levels decreased significantly only in the right ventricle after 8 days. Following a unilateral vagotomy (random, left or right), acetylcholine concentrations in the distal portion of the severed nerve decreased to half the value of the intact contralateral side by 4 days. The continued presence of acetylcholine, despite significantly reduced CAT activity in the severed nerve and transplanted heart, suggests that acetylcholine is preserved, perhaps by vesiculation in nonstimulated postganglionic terminals. The localized decrease in acetylcholine in the right ventricle after 8 days suggests that transplantation may interrupt the postganglionic fibers to this area.
采用液相色谱分析法测定大鼠心脏移植至腹部2天、4天和8天后四个腔室中的胆碱和乙酰胆碱浓度。同时对宿主大鼠的心脏进行相应测量。我们发现，对照组心脏中乙酰胆碱浓度呈现出与胆碱乙酰转移酶（CAT）活性相同的不均匀分布模式，心房中浓度最高（8 - 12 nmol/g），心室中较低（0.7 - 1.6 nmol/g）。移植后，仅在8天后右心室中的乙酰胆碱水平显著下降。单侧迷走神经切断术（随机，左侧或右侧）后，切断神经远端的乙酰胆碱浓度在4天时降至完整对侧的一半。尽管切断神经和移植心脏中的CAT活性显著降低，但乙酰胆碱仍持续存在，这表明乙酰胆碱可能通过非刺激节后终末的囊泡化得以保留。8天后右心室中乙酰胆碱的局部降低表明移植可能中断了该区域的节后纤维。
