Slavíková J, Tucek S
Physiol Bohemoslov. 1985;34(3):217-23.
The activity of choline acetyltransferase (ChAt; E.C. 2.3.1.6) measured as the bromoacetylcholine-sensitive portion of the maximal rate of acetylcholine synthesis has been determined in homogenates of nine regions of the heart of control rats and rats sacrificed 72-74 h after bilateral cervical vagotomy. When related to the content of protein, the activity of ChAT was lowered by 30% in the atria and unchanged in the ventricles of vagotomized rats. The highest absolute decline caused by vagotomy was observed in the sinoatrial region; it was somewhat less in the rest of the right atrium and in the interatrial septum and considerably less in the left atrium. It is concluded that preganglionic parasympathetic fibres supply mainly the sinoatrial region and the right atrium, and that they do not branch to the ventricles. Preganglionic ChAT nts about 30% of total ChAT activity in the atria. The sinoatrio-ventricular gradient in the distribution of ChAT in the heart is due to uneven distribution of both the pre- and postganglionic ChAT pools (i.e., of both the pre- and postganglionic cholinergic nerve fibres and nerve cell bodies).
以乙酰胆碱合成最大速率中对溴乙酰胆碱敏感的部分来衡量的胆碱乙酰转移酶（ChAt；E.C. 2.3.1.6）活性，已在对照大鼠心脏九个区域的匀浆以及双侧颈迷走神经切断术后72 - 74小时处死的大鼠心脏匀浆中测定。与蛋白质含量相关时，迷走神经切断大鼠心房中的ChAT活性降低了30%，心室中的活性未变。迷走神经切断引起的绝对下降幅度在窦房区域最高；在右心房其余部分和房间隔中稍低，在左心房中则低得多。结论是，节前副交感神经纤维主要供应窦房区域和右心房，且它们不分支至心室。节前ChAT约占心房总ChAT活性的30%。心脏中ChAT分布的窦房 - 心室梯度是由于节前和节后ChAT池（即节前和节后胆碱能神经纤维及神经细胞体）分布不均所致。
