Li Zhiting, Zhao Wei, Zhang Jinpeng, Pan Zhiliang, Bai Shengjun, Tong Chunfa
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2022 Apr 12;11(8):1046. doi: 10.3390/plants11081046.
Although the crossover (CO) patterns of different species have been extensively investigated, little is known about the landscape of CO patterns in because of its high heterozygosity and long-time generation. A novel strategy was proposed to reveal the difference of CO rate and interference between and using their F1 hybrid population. We chose restriction site-associated DNA (RAD) tags that contained two SNPs, one only receiving the CO information from the female and the other from the male . These RAD tags allowed us to investigate the CO patterns between the two outbred species, instead of using the traditional backcross populations in inbred lines. We found that the CO rate in was generally greater than that in , and that the CO interference was a common phenomenon across the two genomes. The COs landscape of the different species facilitates not only to understand the evolutionary mechanism for adaptability but also to rebuild the statistical model for precisely constructing genetic linkage maps that are critical in genome assembly in . Additionally, the novel strategy could be applied in other outbred species for investigating the CO patterns.
尽管不同物种的交叉（CO）模式已得到广泛研究，但由于其高杂合性和长时间的世代周期，关于[物种名称]的CO模式情况却知之甚少。有人提出了一种新策略，利用[物种名称]的F1杂交群体来揭示[物种名称1]和[物种名称2]之间CO速率和干扰的差异。我们选择了包含两个单核苷酸多态性（SNP）的限制性位点相关DNA（RAD）标签，其中一个仅接收来自雌性[物种名称1]的CO信息，另一个接收来自雄性[物种名称2]的CO信息。这些RAD标签使我们能够研究这两个远交物种之间的CO模式，而不是使用近交系中的传统回交群体。我们发现，[物种名称1]中的CO速率通常大于[物种名称2]中的CO速率，并且CO干扰是两个基因组中的常见现象。不同[物种名称]的CO图谱不仅有助于理解适应性的进化机制，还有助于重建统计模型，以便精确构建在[物种名称]的基因组组装中至关重要的遗传连锁图谱。此外，这种新策略可应用于其他远交物种以研究CO模式。
