Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), Avenida Presidente Frondizi 2650, 2700, Pergamino, Buenos Aires, Argentina.
Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires (CITNOBA-CONICET), UNNOBA-UNSAdA, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Sci Rep. 2022 Apr 20;12(1):6538. doi: 10.1038/s41598-022-09874-7.
The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D (Rp-BicD) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus, we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus.
最近，随着对 Rhodnius prolixus 基因组的测序，对其发育过程的研究取得了进展。在这项工作中，我们分析了母体基因表达对 R. prolixus 卵子发生和早期胚胎发生的驱动作用。我们检查了 mRNA 的转录谱，以确定在卵巢、未受精卵和 R. prolixus 不同胚胎阶段（产卵后 0、12、24 和 48 小时）表达的基因，直到生殖带原基的形成。我们鉴定了 81 个假定的母体和卵巢相关基因，并通过 qRT-PCR 验证了它们的表达。我们通过原位杂交和亲本 RNAi 验证了同源基因 Bicaudal-D（Rp-BicD）的功能。与在 R. prolixus 的卵子发生和早期发育中的作用一致，我们表明 Rp-BicD 的缺失不会显著影响卵子发生，但会损害胚盘的形成。基于我们的发现，我们提出了母体基因在 R. prolixus 的卵子发生和胚胎发生中的三次作用时间。
