Research & Development, Sanofi, Campus Mérieux, 1541 Avenue Marcel Mérieux, 69280, Marcy L'Etoile, France.
Sci Rep. 2022 Apr 21;12(1):6570. doi: 10.1038/s41598-022-10281-1.
The World Health Organization protocol for rabies post-exposure prophylaxis (PEP) recommends extensive wound washing, immediate vaccination, and administration of rabies immunoglobulin (RIG) in severe category III exposures. Some studies have shown that RIG can interfere with rabies vaccine immunogenicity to some extent. We investigated the interference of RIG on a next generation highly purified Vero cell rabies vaccine candidate (PVRV-NG) versus standard-of-care vaccines in a previously described hamster model. The interference of either human (h) or equine (e) RIG on the immune response elicited by PVRV-NG, Verorab (purified Vero cell rabies vaccine, PVRV), and Imovax Rabies (human diploid cell rabies vaccine; HDCV) was evaluated using the 4-dose Essen PEP regimen. The anti-rabies seroneutralizing titers and specific serum IgM titers were measured by fluorescent antibody virus neutralization test and enzyme-linked immunosorbent assay, respectively, for the vaccines administered with or without RIG. The RIG interference on PVRV-NG, observed transiently at Day 7, was similar to that on PVRV and tended to be lower than that on HDCV using both read-outs. In summary, the results generated in the hamster model showed that RIG induced similar or less interference on PVRV-NG than the standard-of-care vaccines.
世界卫生组织狂犬病暴露后预防（PEP）方案建议在严重的 III 类暴露中进行广泛的伤口清洗、立即接种疫苗和狂犬病免疫球蛋白（RIG）的使用。一些研究表明，RIG 在某种程度上会干扰狂犬病疫苗的免疫原性。我们在先前描述的仓鼠模型中研究了 RIG 对下一代高度纯化的 Vero 细胞狂犬病疫苗候选物（PVRV-NG）与标准护理疫苗的干扰作用。使用 4 剂 Essen PEP 方案评估了 hRIG 或 eRIG 对 PVRV-NG、Verorab（纯化 Vero 细胞狂犬病疫苗，PVRV）和 Imovax Rabies（人二倍体细胞狂犬病疫苗；HDCV）引发的免疫反应的干扰作用。通过荧光抗体病毒中和试验和酶联免疫吸附试验分别测量狂犬病血清中和抗体滴度和特异性血清 IgM 滴度，用于评估与 RIG 联合或不联合使用的疫苗。在第 7 天观察到的对 PVRV-NG 的 RIG 干扰是短暂的，与 PVRV 的干扰相似，并且与两种检测结果相比，对 HDCV 的干扰趋于更低。总之，在仓鼠模型中产生的结果表明，RIG 对 PVRV-NG 的干扰与标准护理疫苗相似或更小。
