Primrose W J, McDonald G A, O'Brien M J, Vaughan C W, Strong M S
Ann Otol Rhinol Laryngol. 1987 Jan-Feb;96(1 Pt 1):47-52. doi: 10.1177/000348948709600111.
The carbon dioxide and neodymium:yttrium aluminum garnet lasers have well documented but characteristically different biological effects, yet little is known about their cumulative, synergistic, or paradoxical effects when used sequentially on living tissue. Using a Merrimack ML 880 laser, a series of superimposed CO2 and Nd:YAG lesions in various combinations were produced on the undersurface of dog tongues. Therapeutic time and power settings were chosen and the number of applications varied, with suitable controls. Observations and measurements were made on acute, healing, and healed lesions. All lesions were excised and submitted for routine hematoxylin and eosin histology. Acute lesions were also assessed for cell viability using rhodamine 123 as a supravital marker. The results show that, even though all the lesions eventually heal, the actual cell damage produced by the Nd:YAG laser is much more than is suggested by the size of the acute lesion. This cell damage can be reduced by the surface carbonization produced by initial application of the CO2 laser. Higher surface temperatures are reached in this combination with less fibrosis and scarring than equal energy counterparts where the Nd:YAG laser was applied first. The knowledge of these synergistic effects can be used to advantage in the clinical setting. The rhodamine 123 technique also appears to be a valid measure of acute thermal tissue injury.
钇铝石榴石激光具有已充分记录但特征不同的生物学效应，然而，当它们依次作用于活体组织时，其累积、协同或矛盾效应却鲜为人知。使用一台梅里马克ML 880激光，在狗舌的下表面产生了一系列不同组合的叠加二氧化碳和钕：钇铝石榴石损伤。选择了治疗时间和功率设置，并改变了照射次数，同时设置了合适的对照。对急性、愈合中和愈合后的损伤进行了观察和测量。所有损伤均被切除并送去进行常规苏木精和伊红组织学检查。还使用罗丹明123作为活体细胞标记物对急性损伤的细胞活力进行了评估。结果表明，尽管所有损伤最终都会愈合，但钕：钇铝石榴石激光产生的实际细胞损伤远大于急性损伤大小所显示的程度。这种细胞损伤可以通过最初使用二氧化碳激光产生的表面碳化来减少。与先使用钕：钇铝石榴石激光的同等能量照射相比，这种组合能达到更高的表面温度，且纤维化和瘢痕形成更少。了解这些协同效应可在临床环境中发挥优势。罗丹明123技术似乎也是急性热组织损伤的一种有效测量方法。
