Raj Savan K, Choudhary Babita, Yadav Anshul, Patidar Rajesh, Mishra Avinash, Kulshrestha Vaibhav
CSIR-Central Salt and Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar 364 002, Gujarat, India.
Department of Physics, The MK Bhavnagar University, Bhavnagar 364 002, Gujarat, India.
Heliyon. 2022 Apr 7;8(4):e09259. doi: 10.1016/j.heliyon.2022.e09259. eCollection 2022 Apr.
Brightly fluorescent Carbon Dots (CDs) were synthesized by green hydrothermal method using commonly available biomass (Aloe vera) as carbon precursor. Their physiochemical and optical characterization was done by standard microscopic and spectroscopic techniques. Photophysical features of their aqueous dispersion were investigated in detail. The influence of wide pH range (2-12), high ionic load (2M) and temperature on their photoluminescence behavior was investigated. Their in-vitro cytotoxicity examination was conducted on Human Cervical Cancer Cells (HeLa) using MTT assay. Testing of their ion-recognition property for common metal ions was done in aqueous medium. These CDs exhibited preferential interaction with Fe over other tested metal ions, without any functionalization. Interaction between CDs and Fe was analyzed in the light of Density Functional Theory (DFT). The work demonstrates that these CDs are acting as nanoprobe for Fe and sensing it at ultra-trace level (5 nM).
采用绿色水热法，以常见生物质（芦荟）为碳前驱体，合成了具有明亮荧光的碳点（CDs）。通过标准的显微镜和光谱技术对其进行了物理化学和光学表征。详细研究了其水分散体的光物理特性。研究了宽pH范围（2 - 12）、高离子负载量（2M）和温度对其光致发光行为的影响。使用MTT法对人宫颈癌细胞（HeLa）进行了体外细胞毒性检测。在水介质中测试了它们对常见金属离子的离子识别性能。这些碳点在未进行任何功能化的情况下，与铁相比其他测试金属离子表现出优先相互作用。根据密度泛函理论（DFT）分析了碳点与铁之间的相互作用。这项工作表明，这些碳点可作为铁的纳米探针，并能在超痕量水平（5 nM）检测铁。
