Department of Pharmaceutical Toxicology, Ankara University Faculty of Pharmacy, Ankara, Turkey.
Drug Chem Toxicol. 2023 May;46(3):523-533. doi: 10.1080/01480545.2022.2066114. Epub 2022 Apr 21.
Traditional two-dimensional (2D) cell culture employed for centuries is extensively used in toxicological studies. There is no doubt that 2D cell culture has made significant contributions to toxicology. However, in today's world, it is necessary to develop more physiologically relevant models. Three-dimensional (3D) cell culture, which can recapitulate the cell's microenvironment, is, therefore, a more realistic model compared to traditional cell culture. In toxicology, 3D cell culture models are a powerful tool for studying different tissues and organs in similar environments and behave as if they are in conditions. In this review, we aimed to present 3D cell culture models that have been used in different organ toxicity studies. We reported the results and interpretations obtained from these studies. We aimed to highlight 3D models as the future of cell culture by reviewing 3D models used in different organ toxicity studies.
传统的二维（2D）细胞培养已经使用了几个世纪，广泛应用于毒理学研究。毫无疑问，2D 细胞培养为毒理学做出了重大贡献。然而，在当今世界，有必要开发更具生理相关性的模型。与传统细胞培养相比，三维（3D）细胞培养可以更好地模拟细胞的微环境，因此是一种更现实的模型。在毒理学中，3D 细胞培养模型是研究不同组织和器官在相似环境中行为的有力工具，就好像它们处于体内条件下一样。在这篇综述中，我们旨在介绍已用于不同器官毒性研究的 3D 细胞培养模型。我们报告了从这些研究中获得的结果和解释。我们旨在通过回顾不同器官毒性研究中使用的 3D 模型，强调 3D 模型作为细胞培养的未来。
