Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.
Acta Neurobiol Exp (Wars). 2022;82(1):106-119. doi: 10.55782/ane-2022-010.
We demonstrate in vivo imaging of the ischemic area in the mouse brain after photostroke using a custom prototype Gaussian‑beam optical coherence tomography (OCT) setup in which the near infrared imaging beam and the green photoinducing light pass through the same objective lens. The goal of our research was analysis of vascularity of the ischemic area during 2‑week progress of stroke and correlating the hypo‑ and hyperreflective OCT scattering areas with the location of activated microglia and astroglia. Angiogenesis, which was assessed using angiomaps, showed that the area of vessels in the ischemic center increased until day 7. OCT imaging revealed a heterogeneous scattering signal pattern in the ischemic area. On structural OCT images, we found presence of a core area of ischemia with a hyporeflective OCT signal and a halo of hyperreflective signal around the core. The core signal decreased in size by 70% by day 14. Immunocytochemistry revealed that the hyporeflective area in the ischemic core was associated with microglia/macrophage activation, whereas the hyperreflective signal from the halo came from activated astrocytes.
我们使用定制的高斯光束光学相干断层扫描 (OCT) 原型设备在活体中对光诱导脑卒中后小鼠大脑的缺血区域进行成像，该设备中近红外成像光束和绿色光致发光诱导光通过相同的物镜。我们的研究目标是分析在脑卒中 2 周进展过程中缺血区域的血管生成情况，并将低反射和高反射 OCT 散射区域与激活的小胶质细胞和星形胶质细胞的位置相关联。使用血管图评估的血管生成表明，缺血中心的血管面积增加直到第 7 天。OCT 成像显示在缺血区域存在异质散射信号模式。在结构 OCT 图像上，我们发现缺血核心区域存在低反射 OCT 信号的核心区域和核心周围的高反射信号晕。到第 14 天，核心信号的大小减小了 70%。免疫细胞化学显示，缺血核心的低反射区域与小胶质细胞/巨噬细胞激活有关，而晕环的高反射信号来自激活的星形胶质细胞。
