IEEE Trans Biomed Eng. 2018 Oct;65(10):2136-2142. doi: 10.1109/TBME.2017.2706976. Epub 2017 May 23.
Monitoring hemodynamic and vascular changes in the acute stages of mouse stroke models is invaluable in studying ischemic stroke pathophysiology. However, there lacks a tool to simultaneously and dynamically investigate these changes.
We integrated laser speckle imaging (LSI) and visible-light optical coherence tomography (Vis-OCT) to reveal dynamic vascular responses in acute stages in the distal middle cerebral artery occlusion (dMCAO) model in rodents. LSI provides full-field, real-time imaging to guide Vis-OCT imaging and monitor the dynamic cerebral blood flow (CBF). Vis-OCT offers depth-resolved angiography and oxygen saturation (sO2) measurements.
Our results showed detailed CBF and vasculature changes before, during, and after dMCAO. After dMCAO, we observed insignificant sO2 variation in arteries and arterioles and location-dependent sO2 drop in veins and venules. We observed that higher branch-order veins had larger drops in sO2 at the reperfusion stage after dMCAO.
This work suggests that integrated LSI and Vis-OCT is a promising tool for investigating ischemic stroke in mouse models.
For the first time, LSI and Vis-OCT are integrated to investigate ischemic strokes in rodent models.
监测小鼠中风模型急性阶段的血液动力学和血管变化，对于研究缺血性中风病理生理学非常有价值。然而，目前缺乏一种能够同时动态研究这些变化的工具。
我们将激光散斑成像（LSI）和可见光光学相干断层扫描（Vis-OCT）集成在一起，以揭示啮齿动物远端大脑中动脉闭塞（dMCAO）模型中急性阶段的动态血管反应。LSI 提供全场实时成像，以指导 Vis-OCT 成像并监测动态脑血流（CBF）。Vis-OCT 提供深度分辨血管造影和氧饱和度（sO2）测量。
我们的结果显示了 dMCAO 前后详细的 CBF 和血管变化。在 dMCAO 后，我们观察到动脉和小动脉中 sO2 变化不明显，而静脉和小静脉中 sO2 位置依赖性下降。我们观察到，在 dMCAO 后再灌注阶段，较高分支阶数的静脉 sO2 下降幅度更大。
这项工作表明，集成的 LSI 和 Vis-OCT 是研究小鼠模型中缺血性中风的有前途的工具。
首次将 LSI 和 Vis-OCT 集成在一起，用于研究啮齿动物模型中的缺血性中风。
