Wu Q Y, Drouet L, Carrier J L, Rothschild C, Berard M, Rouault C, Caen J P, Meyer D
Arteriosclerosis. 1987 Jan-Feb;7(1):47-54. doi: 10.1161/01.atv.7.1.47.
Endothelial cells were cultured from the thoracic aorta, inferior vena cava, and pulmonary artery of normal adult and young pigs as well as from pigs with von Willebrand disease (vWD). The von Willebrand factor (vWF) was estimated by ELISA in endothelial cell supernatants and lysates as well as by immunofluorescence of cells by use of either a polyclonal or a monoclonal antibody to vWF. In normal adult pigs, the content of vWF in supernatants and cell lysates was the highest in the pulmonary artery, lower in the inferior vena cava, and almost nil in the thoracic aorta. In the normal young pigs, vWF was higher in the inferior vena cava endothelial cell supernatants and lysates than in the pulmonary artery. Thus the synthesis of vWF by endothelial cells varies along the vascular tree and appears to be modulated by the age of the animals. In pigs with vWD, levels of vWF were slightly detectable in endothelial cells from the pulmonary artery (contrasting with levels of plasmatic vWF below 0.01 U/ml), but were undetectable in the thoracic aorta and inferior vena cava. Thus, if vWF plays a role in atherogenesis, this involves circulating, not its intracellular, form.
从正常成年猪和幼猪以及患血管性血友病（vWD）的猪的胸主动脉、下腔静脉和肺动脉中培养内皮细胞。通过ELISA法检测内皮细胞上清液和裂解物中的血管性血友病因子（vWF），并使用针对vWF的多克隆或单克隆抗体通过细胞免疫荧光法进行检测。在正常成年猪中，上清液和细胞裂解物中vWF的含量在肺动脉中最高，在下腔静脉中较低，在胸主动脉中几乎为零。在正常幼猪中，下腔静脉内皮细胞上清液和裂解物中的vWF高于肺动脉。因此，内皮细胞合成vWF的情况沿血管树而异，并且似乎受动物年龄的调节。在患vWD的猪中，肺动脉内皮细胞中可略微检测到vWF水平（与血浆vWF水平低于0.01 U/ml形成对比），但在胸主动脉和下腔静脉中未检测到。因此，如果vWF在动脉粥样硬化形成中起作用，这涉及循环形式而非细胞内形式的vWF。
