Ragelle Héloïse, Dernick Karen, Westenskow Peter D, Kustermann Stefan
Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Methods Mol Biol. 2022;2475:239-257. doi: 10.1007/978-1-0716-2217-9_18.
Relevant human in vitro models of the retinal microvasculature can be used to study the role of disease mediators on retinal barrier dysfunction and assess the efficacy of early drug candidates. This chapter describes an organ-on-a-chip model of the retinal microvasculature that allows for facile quantification of barrier permeability in response to leakage mediators, such as Vascular Endothelial Growth Factor (VEGF), and enables screening of VEGF-induced permeability inhibitors. This chapter also presents an automated confocal imaging method for the visualization of endothelial tube morphology as an additional measure of barrier integrity.
视网膜微血管的相关人体体外模型可用于研究疾病介质对视网膜屏障功能障碍的作用，并评估早期候选药物的疗效。本章介绍了一种视网膜微血管芯片器官模型，该模型能够轻松量化对诸如血管内皮生长因子（VEGF）等渗漏介质的屏障通透性反应，并能够筛选VEGF诱导的通透性抑制剂。本章还介绍了一种自动共聚焦成像方法，用于可视化内皮管形态，作为屏障完整性的一项额外指标。
