胚胎期小鼠后脑和出生后视网膜作为研究血管生成的体内模型。

The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis.

作者信息

Fantin Alessandro, Ruhrberg Christiana

机构信息

Department of Biosciences, University of Milan, Milan, Italy.

UCL Institute of Ophthalmology, University College London, London, UK.

出版信息

Methods Mol Biol. 2022;2475:275-287. doi: 10.1007/978-1-0716-2217-9_20.

DOI:10.1007/978-1-0716-2217-9_20

PMID:35451765

Abstract

Angiogenesis, the growth of new blood vessels from pre-existing ones, is a fundamental process for organ development, exercise-induced muscle growth, and wound healing, but is also associated with different diseases such as cancer and neovascular eye disease. Accordingly, elucidating the molecular and cellular mechanisms of angiogenesis has the potential to identify new therapeutic targets to stimulate new vessel formation in ischemic tissues or inhibit pathological vessel growth in disease. This chapter describes the mouse embryo hindbrain and postnatal retina as models to study physiological angiogenesis and provides detailed protocols for tissue dissection, sample staining and analysis.

摘要

血管生成，即从已有的血管中生长出新的血管，是器官发育、运动诱导的肌肉生长和伤口愈合的一个基本过程，但也与癌症和新生血管性眼病等不同疾病相关。因此，阐明血管生成的分子和细胞机制有可能识别出新的治疗靶点，以刺激缺血组织中的新血管形成或抑制疾病中的病理性血管生长。本章将小鼠胚胎后脑和出生后视网膜作为研究生理性血管生成的模型进行描述，并提供了组织解剖、样本染色和分析的详细方案。