鉴定.中的 CRISPR-Cas 系统
Characterization of CRISPR-Cas systems in .
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China.
School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
出版信息
Microb Genom. 2022 Apr;8(4). doi: 10.1099/mgen.0.000812.
The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in , the occurrence and diversity of CRISPR-Cas systems were analysed in 150 strains. Specifically, 47 % (71/150) of genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in , and the protospacer adjacent motif sequences were further characterized in type I-C system as 5'-TTC-3'. All these results might provide a molecular basis for the development of endogenous genome editing tools in .
簇状规律间隔短回文重复（CRISPR）-CRISPR 相关蛋白（Cas）系统是细菌抵抗外源 DNA 感染的一种重要的适应性免疫系统，已被广泛应用于基因分型和基因编辑。为了为 CRISPR-Cas 系统在 中的应用提供理论依据，对 150 株 中的 CRISPR-Cas 系统的发生和多样性进行了分析。具体来说，47%（71/150）的 基因组具有 CRISPR-Cas 系统，其中 I 型-CRISPR-Cas 系统在这些菌株中分布最广。 中存在的间隔序列可用作基因分型标记。此外，I 型-CRISPR-Cas 系统中的噬菌体组装相关蛋白是 的重要靶标，并且进一步将 I 型-C 系统中的原间隔序列邻近基序序列表征为 5'-TTC-3'。所有这些结果可能为开发 中的内源性基因组编辑工具提供分子基础。
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