Di Tenggang, Zhang Huan, Zhang Cheng, Tian Liming, Chang Menghan, Han Wei, Qiao Ruiming, Li Ming, Zhang Shuhong, Yang Guangli
College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China.
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Microorganisms. 2025 Jul 16;13(7):1666. doi: 10.3390/microorganisms13071666.
is a predominant probiotic in animals that is associated with host intestinal health. The protective mechanisms of the () strain, specifically those related to functional gene-host interactions in intestinal homeostasis, remain poorly elucidated. This study reports the complete genome sequence and characterization of a strain isolated from wild pig feces, which comprised a single circular chromosome (1,944,022 bp; GC content 60.49%) with 1567 protein-coding genes, and the strain had certain acid resistance, bile salt resistance, gastrointestinal fluid tolerance, and antibacterial characteristics. Genomic annotation revealed three putative genomic islands and two CRISPR-Cas systems. Functional characterization identified genes encoding carbohydrate-active enzymes (CAZymes) and associated metabolic pathways, indicating that this strain can degrade complex dietary carbohydrates and synthesize bioactive metabolites for gut homeostasis. Although the antibiotic resistance genes were predicted, phenotypic assays demonstrated discordant resistance patterns, indicating complex regulatory networks. This study indicated the genomic basis of -host crosstalk in intestinal protection, providing a framework for developing novel probiotic interventions.
是动物体内的一种主要益生菌，与宿主肠道健康相关。（）菌株的保护机制，特别是那些与肠道稳态中功能基因 - 宿主相互作用相关的机制，仍未得到充分阐明。本研究报告了从野猪粪便中分离出的一株菌株的完整基因组序列和特征，该菌株包含一条单环状染色体（1,944,022 bp；GC含量60.49%），有1567个蛋白质编码基因，并且该菌株具有一定的耐酸性、耐胆盐性、胃肠道液耐受性和抗菌特性。基因组注释揭示了三个假定的基因组岛和两个CRISPR - Cas系统。功能表征鉴定出编码碳水化合物活性酶（CAZymes）和相关代谢途径的基因，表明该菌株可以降解复杂的膳食碳水化合物并合成生物活性代谢物以维持肠道稳态。虽然预测了抗生素抗性基因，但表型分析显示出不一致的抗性模式，表明存在复杂的调控网络。本研究揭示了肠道保护中 - 宿主相互作用的基因组基础，为开发新型益生菌干预措施提供了框架。
