Mathialagan N, Rao A J
Biochem Int. 1986 Nov;13(5):757-65.
An in vitro system using the minces of placental villi from first trimester human pregnancy (6-10 weeks) has been validated to examine the effect of addition of GnRH and its analogues on hCG secreted into the medium. Addition of low concentration of GnRH or its analogues (1 X 10(-8) M to 1 X 10(-6) M) resulted in an increase in the quantity of hCG in the medium, while addition of high concentrations of GnRH resulted in an inhibitory effect. Of the analogues tested, Buserelin was highly effective in exerting an inhibitory effect. A significant increase in 35S-methionine incorporation into immunoprecipitable hCG was noticed in the presence of GnRH. These results suggests that GnRH stimulates both synthesis and secretion of hCG by first trimester human placenta.
一种使用妊娠早期（6 - 10周）人胎盘绒毛匀浆的体外系统已得到验证，用于研究添加促性腺激素释放激素（GnRH）及其类似物对培养基中分泌的人绒毛膜促性腺激素（hCG）的影响。添加低浓度的GnRH或其类似物（1×10⁻⁸ M至1×10⁻⁶ M）导致培养基中hCG量增加，而添加高浓度的GnRH则产生抑制作用。在所测试的类似物中，布舍瑞林在发挥抑制作用方面非常有效。在存在GnRH的情况下，观察到35S - 甲硫氨酸掺入免疫沉淀的hCG中有显著增加。这些结果表明，GnRH刺激妊娠早期人胎盘hCG的合成和分泌。
