Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
Exploratory Research Center on Life and Living Systems (ExCELLS), 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787, Japan.
Glycobiology. 2022 Jul 13;32(8):646-650. doi: 10.1093/glycob/cwac025.
High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web application Glycoanalysis by the Three Axes of MS and Chromatography (GALAXY), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Herein, we describe the updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multiomics data.
高效液相色谱（HPLC）洗脱数据为定量糖基化分析提供了一种有用的工具，可用于区分异构寡糖。基于 Noriko Takahashi 博士开发的 N 连接糖基化的吡啶-2-氨基化的三维 HPLC 图谱的网络应用程序“MS 和色谱的三个轴的糖基分析（GALAXY）”已广泛用于分子、细胞和组织水平的 N-糖基化分析。在这里，我们将更新后的 GALAXY 描述为版本 3，它包括新的 HPLC 数据，包括葡萄糖醛酸化和硫酸化聚糖，使用现代技术改进的图形用户界面，以及与 GlyTouCan 和 GlyCosmos 门户中的聚糖信息的链接。这种联系将促进与多组学数据一起对人类和其他生物体进行糖组学分析。
