LINC00342 通过靶向 miR-15b/TPBG 诱导肺腺癌转移。
LINC00342 induces metastasis of lung adenocarcinoma by targeting miR-15b/TPBG.
机构信息
Department of Cardiothoracic Surgery, Sanya People's Hospital; West China (Sanya) Hospital of Sichuan University, Sanya, PR China.
Department of Cardiothoracic Surgery, Hebei Province Hospital of Chinese Medicine, Shijiazhuang, PR China.
出版信息
Acta Biochim Pol. 2022 Apr 22;69(2):291-297. doi: 10.18388/abp.2020_5697.
In this study, the function and regulation of long non-coding RNA (lncRNA) LINC00342 in lung adenocarcinoma were investigated. From The Cancer Genome Atlas (TCGA) datasets and Gene Expression Omnibus (GEO) datasets, LINC00342 was found to be up-regulated in lung adenocarcinoma. The high expression of LINC00342 was also validated in lung cancer cell lines. LINC00342 induced invasion and epithelial-mesenchymal transition (EMT) process of A549 cells. By analyzing GEO datasets, TPBG was confirmed positively correlated to LINC00342 and highly expressed in lung adenocarcinoma. In addition, TPBG induced invasion and EMT process of A549 cells. Through bioinformatics analysis and luciferase assay, miR-15b was validated as a direct target of both LINC00342 and TPBG. Ectopic miR-15 expression repressed LINC00342 and TPBG. Interestingly, LINC00342 overexpression inhibited miR-15b and induced TPBG, whereas ectopic TPBG unchanged LINC00342 and miR-15b levels. In conclusion, LINC00342 promotes metastasis of lung adenocarcinoma through inducing TPBG targeted by miR-15b.
在这项研究中，研究了长链非编码 RNA（lncRNA）LINC00342 在肺腺癌中的功能和调节作用。从癌症基因组图谱（TCGA）数据集和基因表达综合数据库（GEO）数据集中发现，LINC00342 在肺腺癌中上调。LINC00342 的高表达也在肺癌细胞系中得到了验证。LINC00342 诱导 A549 细胞的侵袭和上皮间质转化（EMT）过程。通过分析 GEO 数据集，确认 TPBG 与 LINC00342 呈正相关，并在肺腺癌中高表达。此外，TPBG 诱导 A549 细胞的侵袭和 EMT 过程。通过生物信息学分析和荧光素酶报告基因实验，验证了 miR-15b 是 LINC00342 和 TPBG 的直接靶标。过表达 miR-15b 抑制 LINC00342 和 TPBG。有趣的是，LINC00342 过表达抑制 miR-15b 并诱导 TPBG，而外源性 TPBG 对 LINC00342 和 miR-15b 水平没有影响。总之，LINC00342 通过靶向 miR-15b 的 TPBG 促进肺腺癌的转移。
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