Laboratory of Anaesthesiology & Critical Care Medicine, Department of Anesthesiology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
Medical College, Tibet University, Lhasa 850000, China.
Anal Chem. 2022 May 3;94(17):6441-6445. doi: 10.1021/acs.analchem.2c00460. Epub 2022 Apr 22.
As a neurotransmitter, norepinephrine (NE) is critical for psychiatric conditions, neurodegenerative diseases, and pheochromocytoma. A real-time and noninvasive method for the detection of NE as a tracer to investigate the NE-relevant disease treatment process is urgently desirable. Herein, we successfully developed a turn-on NE bioluminescent probe (NBP), which was grounded on -toluenethiol deprotectrf by nucleophilic substitution. Compared with other analytes, the NBP exhibited high sensitivity and selectivity . More importantly, the NBP provides a promising strategy for imaging of NE in living animals with noninvasive visualization and real-time features.
作为一种神经递质，去甲肾上腺素（NE）对精神疾病、神经退行性疾病和嗜铬细胞瘤至关重要。因此，人们迫切需要一种实时、非侵入性的方法，使用 NE 作为示踪剂来检测 NE 相关疾病的治疗过程。在此，我们成功开发了一种基于 -甲苯硫醇去保护的 NE 生物发光探针（NBP），该探针通过亲核取代反应进行检测。与其他分析物相比，NBP 具有高灵敏度和选择性。更重要的是，NBP 为活体动物中 NE 的成像提供了一种很有前途的策略，具有非侵入性可视化和实时检测的特点。
