Mostafa Islam Mohamed, Abdussalam Abubakar, Zholudov Yuriy Tymofiiovych, Snizhko Dmytro Viktorovych, Zhang Wei, Hosseini Morteza, Guan Yiran, Xu Guobao
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
University of Science and Technology of China, Hefei 230000, P.R. China.
Chem Biomed Imaging. 2023 Apr 13;1(4):297-314. doi: 10.1021/cbmi.2c00002. eCollection 2023 Jul 24.
Imaging technologies based on chemiluminescence (CL) and bioluminescence (BL) have seen a tremendous growth in the past decade due to their extensive contributions to biochemical analysis and biomedical science. CL and BL imaging technologies have many advantages over commonly used imaging techniques (such as fluorescence and electrochemical systems). CL and BL proceed without an external light source, thus avoiding photobleaching, high interference of background signal, and autofluorescence. Furthermore, CL and BL analytical techniques are characterized by their low detection limit, high selectivity, short assay time, and simple instrumentation. Recently, CL-based imaging technology has been applied successfully for the determination and imaging of several significant analytes. Meanwhile, an innovative BL imaging technology has been established for the noninvasive real-time tracking of different biomolecules relying on the interaction between luciferase and its substrate. In the current review, we discuss the recent applications of CL and BL imaging approaches over the past five years. Finally, we also discuss the current state of progress and the prospects for CL and BL imaging systems.
基于化学发光（CL）和生物发光（BL）的成像技术在过去十年中取得了巨大的发展，因为它们对生化分析和生物医学科学做出了广泛贡献。与常用的成像技术（如荧光和电化学系统）相比，CL和BL成像技术具有许多优势。CL和BL成像无需外部光源，从而避免了光漂白、背景信号的高干扰和自发荧光。此外，CL和BL分析技术具有检测限低、选择性高、分析时间短和仪器简单的特点。最近，基于CL的成像技术已成功应用于几种重要分析物的测定和成像。同时，一种创新的BL成像技术已经建立，用于基于荧光素酶与其底物之间的相互作用对不同生物分子进行无创实时跟踪。在本综述中，我们讨论了CL和BL成像方法在过去五年中的最新应用。最后，我们还讨论了CL和BL成像系统的当前进展情况和前景。
