Tolentino Miguel, Cho Chi C, Lyons Jeri-Anne
Biomedical Sciences, University of Wisconsin Milwaukee, Milwaukee, Wisconsin, USA.
College of Health Sciences, University of Wisconsin Milwaukee, Milwaukee, Wisconsin, USA.
Photobiomodul Photomed Laser Surg. 2022 Apr;40(4):234-244. doi: 10.1089/photob.2021.0169.
Photobiomodulation (PBM) therapy was previously shown to reduce the clinical severity of disease and modulated pro- and anti-inflammatory cytokines in an animal model of multiple sclerosis (MS). Previous observations were extended to determine the effect of PBM therapy on peripheral blood mononuclear cells and CD4 T cells isolated from persons with MS (PwMS) and healthy donors. Using an cell culture system, isolated cells were activated and treated with red or near-infrared light wavelengths to determine the effect of PBM on the production of interferon gamma and interleukin-10 (IL-10). PBM modulated cytokine production in MS subjects and healthy donors in a dose- and wavelength-dependent manner, with MS subjects and healthy donors responding differently to administered light. In addition, disease severity affected the response of immune cells, for instance, 670 nm increased IL-10 production associated with increased disease severity. The data show that PBM therapy has the potential to modulate pro- and anti-inflammatory cytokines in PwMS over the course of disease. Further experiments applying PBM treatment directly on patients should be carried out with extreme caution to avoid severe imbalance in the immune response.
先前的研究表明，光生物调节（PBM）疗法可减轻疾病的临床严重程度，并在多发性硬化症（MS）动物模型中调节促炎和抗炎细胞因子。先前的观察结果得到了扩展，以确定PBM疗法对从MS患者（PwMS）和健康供体中分离出的外周血单个核细胞和CD4 T细胞的影响。使用细胞培养系统，将分离出的细胞激活并用红色或近红外光波长进行处理，以确定PBM对干扰素γ和白细胞介素-10（IL-10）产生的影响。PBM以剂量和波长依赖性方式调节MS患者和健康供体中的细胞因子产生，MS患者和健康供体对所给予的光反应不同。此外，疾病严重程度影响免疫细胞的反应，例如，670nm增加了与疾病严重程度增加相关的IL-10产生。数据表明，PBM疗法有可能在疾病过程中调节PwMS中的促炎和抗炎细胞因子。对患者直接应用PBM治疗的进一步实验应极其谨慎地进行，以避免免疫反应严重失衡。
