University of the Basque Country (UPV/EHU), Faculty of Pharmacy, Department of Zoology and Animal Cell Biology: Paseo de la Universidad, 7. 01006 Vitoria-Gasteiz, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Álava, Spain.
University of the Basque Country (UPV/EHU), Faculty of Pharmacy, Department of Zoology and Animal Cell Biology: Paseo de la Universidad, 7. 01006 Vitoria-Gasteiz, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Álava, Spain.
Mol Phylogenet Evol. 2022 Jul;172:107480. doi: 10.1016/j.ympev.2022.107480. Epub 2022 Apr 19.
Chondrina Reichenbach, 1828 is a highly diverse genus of terrestrial molluscs currently including 44 species with about 28 subspecific taxa. It is distributed through North Africa, central and southern Europe, from Portugal in the West to the Caucasus and Asia Minor in the East. Approximately 70% of the species are endemic to the Iberian Peninsula constituting its main center of speciation with 34 species. This genus includes many microendemic taxa, some of them not yet described, confined to limestone habitats (being strictly rock-dwelling species). They are distributed on rocky outcrops up to 2000 m.a.s.l. It is a genus of conical-fusiform snails that differ mainly in shell characters and in the number and position of teeth in their aperture. So far, molecular studies on Chondrina have been based exclusively on the mitochondrial Cytochrome Oxidase subunit I region (COI). These studies gave a first view of the phylogeny of the genus but many inner nodes were not statistically supported. The main objective of the study is to obtain a better understanding of the phylogeny and systematics of the genus Chondrina on the Iberian Peninsula, using multilocus molecular analysis. Partial sequences of the COI and 16S rRNA genes, as well as of the nuclear Internal Transcribed Spacer 1 (ITS1-5.8S) and Internal Transcribed Spacer 2 (5.8S-ITS2-28S) were obtained from individuals of all the extant Chondrina species known from the Iberian Peninsula. In addition to this, the newly obtained COI sequences were combined with those previously published in the GenBank. Phylogenetic relationships were inferred using maximum likelihood and Bayesian methods. The reconstructed phylogenies showed high values of support for more recent branches and basal nodes. Moreover, molecular species delimitation allowed to better definethe studied species and check the presence of new taxa.
软骨蜗牛属（Chondrina Reichenbach, 1828）是陆生软体动物的一个高度多样化的属，目前包括 44 种，约有 28 个亚种。它分布于北非、中欧和南欧，从西部的葡萄牙到东部的高加索和小亚细亚。大约 70%的物种是伊比利亚半岛的特有种，构成了其主要的物种形成中心，有 34 种。该属包括许多微地方性种群，其中一些尚未被描述，局限于石灰岩栖息地（是严格的岩石居住物种）。它们分布在高达 2000 米海拔的岩石露头处。这是一个圆锥形蜗牛属，主要区别在于壳的特征和口盖中牙齿的数量和位置。到目前为止，软骨蜗牛的分子研究仅基于线粒体细胞色素氧化酶亚基 I 区（COI）。这些研究首次揭示了该属的系统发育，但许多内部节点在统计学上没有得到支持。本研究的主要目的是利用多基因座分子分析，更好地了解伊比利亚半岛软骨蜗牛属的系统发育和分类学。从伊比利亚半岛已知的所有现存软骨蜗牛属个体中获得了 COI 和 16S rRNA 基因的部分序列，以及核内转录间隔区 1（ITS1-5.8S）和内转录间隔区 2（5.8S-ITS2-28S）。此外，新获得的 COI 序列与 GenBank 中先前发表的序列相结合。使用最大似然法和贝叶斯法推断系统发育关系。重建的系统发育树显示，更近的分支和基部节点得到了高度支持。此外，分子物种鉴定允许更好地定义研究物种，并检查新分类群的存在。
