Cruz-Carrión Álvaro, Calani Luca, de Azua Ma Josefina Ruiz, Mena Pedro, Del Rio Daniele, Suárez Manuel, Arola-Arnal Anna
Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Nutrigenomics Research Group, 43007 Tarragona, Spain.
Department of Food and Drugs, University of Parma, 43124 Parma, Italy.
Food Chem. 2022 Sep 15;388:132984. doi: 10.1016/j.foodchem.2022.132984. Epub 2022 Apr 15.
The aim of this work was to address whether the growing location of tomato could generate a different (poly)phenol profile able to affect both in vivo absorption and (poly)phenol metabolite pattern upon tomato consumption. uHPLC-MS analyses allowed to obtain a detailed (poly)phenol profile of tomatoes from two locations in Spain, quantifying 57 (poly)phenolic compounds. However, local and non-local tomatoes showed a different concentration of their native (poly)phenols, which could be attributed to diverse cultivation origin. Rat serum was analysed after an acute tomato feeding. Seven phenolic metabolites were quantified through uHPLC-MS. Pharmacokinetic parameters were further evaluated, revealing different serum concentrations of (poly)phenolic metabolites between tomatoes. The maximum peak serum concentrations, reached mainly after 2 h after ingestion, led to suppose that serum metabolites were mostly derived from absorption in the upper gastrointestinal tract. The growing location of tomatoes affected both the content of native (poly)phenols and their in vivo absorption.
这项工作的目的是研究番茄的种植地点是否会产生不同的（多）酚类物质谱，从而影响番茄食用后的体内吸收以及（多）酚类代谢物模式。超高效液相色谱-质谱联用（uHPLC-MS）分析能够详细获取来自西班牙两个地点的番茄的（多）酚类物质谱，对57种（多）酚类化合物进行了定量分析。然而，本地番茄和非本地番茄的天然（多）酚类物质浓度不同，这可能归因于不同的种植来源。在大鼠急性喂食番茄后对其血清进行了分析。通过uHPLC-MS对七种酚类代谢物进行了定量分析。进一步评估了药代动力学参数，结果显示不同番茄之间的（多）酚类代谢物血清浓度存在差异。血清最大峰值浓度主要在摄入后2小时达到，这表明血清代谢物大多来源于上消化道的吸收。番茄的种植地点既影响天然（多）酚类物质的含量，也影响其体内吸收。
