Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Jinan, China; Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Neuropeptides. 2022 Jun;93:102246. doi: 10.1016/j.npep.2022.102246. Epub 2022 Apr 9.
Neuropeptide Y (NPY) is a significant neuromodulator implicated in a multitude of physiological functions via activating NPY receptors which belong to seven transmembrane G-protein-coupled receptors (GPCRs). However, the detailed cellular expression of NPY receptors in retina has been scarcely investigated. In this study, the expression of the special NPY4R receptor in rat retina was assessed using Western blot analysis and immunofluorescent staining. The detailed cellular localization of NPY4R receptor was studied using double immunofluorescent staining and laser-scanning confocal microscopy. Our data demonstrated that NPY4R receptor was weakly expressed in the inner segment of outer photoreceptors and extensively expressed in the outer segment of S-opsin-positive blue cones, L/M-opsin-positive red/green cones and in the somata of CB-positive horizontal cells, GAD65-positive GABAnergic amacrine cells, ChAT-positive cholinergic amacrine cells, TH-positive dopaminergic CA1 amacrine cells and CA2 amacrine cells, PV-positive AII amacrine cells, Brn3a-positive conventional ganglion cells and melanopsin-containing ipRGCs. In addition, NPY4R receptor was diffusely distributed throughout the full thickness of the inner plexiform layer and outer plexiform layer. However, the outer segment of Rho4D2-positive rods, the somata of ChX10-positive bipolar cells and CRALBP-positive Müller glial cells seemed to lack immunoreactivity of NPY4R receptor. The new finding that multiple types of retinal cell express NPY4R receptor provides new neurobiological basis for the participation of NPY in the regulation of retinal functions through activating NPY4R receptor.
神经肽 Y（NPY）是一种重要的神经调质，通过激活属于七次跨膜 G 蛋白偶联受体（GPCR）的 NPY 受体，参与多种生理功能。然而，NPY 受体在视网膜中的详细细胞表达尚未得到充分研究。在这项研究中，使用 Western blot 分析和免疫荧光染色评估了大鼠视网膜中特殊的 NPY4R 受体的表达。使用双重免疫荧光染色和激光扫描共聚焦显微镜研究了 NPY4R 受体的详细细胞定位。我们的数据表明，NPY4R 受体在内节的外光感受器中弱表达，在外节的 S-opsin 阳性蓝锥体细胞、L/M-opsin 阳性红/绿锥体细胞以及 CB 阳性水平细胞、GAD65 阳性 GABA 能无长突细胞、ChAT 阳性胆碱能无长突细胞、TH 阳性多巴胺能 CA1 无长突细胞和 CA2 无长突细胞、PV 阳性 AII 无长突细胞、Brn3a 阳性常规神经节细胞和含有 melanopsin 的 ipRGCs 中广泛表达。此外，NPY4R 受体在整个内丛状层和外丛状层的全厚度中弥散分布。然而，Rho4D2 阳性杆状细胞的外节、ChX10 阳性双极细胞的体部和 CRALBP 阳性 Müller 胶质细胞似乎缺乏 NPY4R 受体的免疫反应性。新发现多种类型的视网膜细胞表达 NPY4R 受体，为 NPY 通过激活 NPY4R 受体参与调节视网膜功能提供了新的神经生物学基础。
