González Natalia, Abdellati Saïd, De Baetselier Irith, Laumen Jolein Gyonne Elise, Van Dijck Christophe, de Block Tessa, Kenyon Chris, Manoharan-Basil Sheeba Santhini
STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Clinical Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Antibiotics (Basel). 2022 Apr 8;11(4):499. doi: 10.3390/antibiotics11040499.
Emerging resistance to ceftriaxone and azithromycin has led to renewed interest in using ciprofloxacin to treat . This could lead to the rapid emergence and spread of ciprofloxacin resistance. Previous studies investigating the emergence of fluoroquinolone resistance have been limited to a single strain of . It is unknown if different genetic backgrounds affect the evolution of fluoroquinolone resistance in , as has been shown in other bacterial species. This study evaluated the molecular pathways leading to ciprofloxacin resistance in two reference strains of -WHO-F and WHO-P. Three clones of each of the two strains of were evolved in the presence of ciprofloxacin, and isolates from different time points were whole-genome sequenced. We found evidence of strain-specific differences in the emergence of ciprofloxacin resistance. Two out of three clones from WHO-P followed the canonical pathway to resistance proceeding via substitutions in GyrA-S91F, GyrA-D95N and ParC. None of the three WHO-F clones followed this pathway. In addition, mutations in , and frequently occurred in WHO-F clones, whereas mutations in , and occurred in WHO-P.
对头孢曲松和阿奇霉素新出现的耐药性使得人们重新关注使用环丙沙星进行治疗。这可能导致环丙沙星耐药性的迅速出现和传播。以往关于氟喹诺酮耐药性出现的研究仅限于单一菌株。与其他细菌物种的情况一样，不同的遗传背景是否会影响氟喹诺酮耐药性在[具体物种名称未明确]中的演变尚不清楚。本研究评估了导致世界卫生组织（WHO）-F和WHO-P这两种[具体物种名称未明确]参考菌株对环丙沙星耐药的分子途径。在环丙沙星存在的情况下，使这两种[具体物种名称未明确]菌株的三个克隆发生进化，并对不同时间点的分离株进行全基因组测序。我们发现了环丙沙星耐药性出现过程中菌株特异性差异的证据。来自WHO-P的三个克隆中有两个遵循通过GyrA-S91F、GyrA-D95N和ParC中的替换产生耐药性的经典途径。WHO-F的三个克隆均未遵循此途径。此外，[相关基因未明确]、[相关基因未明确]和[相关基因未明确]中的突变在WHO-F克隆中频繁出现，而[相关基因未明确]、[相关基因未明确]和[相关基因未明确]中的突变则出现在WHO-P中。
