Goulart Debora Brito, Beyi Ashenafi Feyisa, Wu Zuowei, Adiguzel Mehmet Cemal, Schroeder Anastasia, Singh Kritika, Xu Changyun, Ocal Melda Meral, Dewell Renee, Dewell Grant A, Plummer Paul J, Zhang Qijing, Sahin Orhan
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Center for Food Security and Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Antibiotics (Basel). 2022 Apr 15;11(4):531. doi: 10.3390/antibiotics11040531.
is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in , 30 commercial calves were divided into Group 1, Group 2, and Group 3 ( = 10), and were all inoculated orally with FQ-susceptible (FQ-S) ; seven days later, Group 3 was challenged with transtracheal , and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R via culture. Before oral inoculation with , 87% of calves were naturally colonized by FQ-R . Two days after the inoculation, FQ-R decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R populations following the injection was due mainly to enrichment of preexisting FQ-R , rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant in cattle in response to FQ treatment.
是食源性肠胃炎的主要病因。最近的研究表明，在使用氟喹诺酮（FQ）控制牛呼吸道疾病（BRD）的牛群中，耐氟喹诺酮（FQ-R）的情况有所增加。为了评估达氟沙星治疗对牛群中FQ耐药性发展的影响，将30头商业犊牛分为第1组、第2组和第3组（每组n = 10），所有犊牛均口服接种FQ敏感（FQ-S）的[病原体名称未给出]；7天后，第3组经气管接种[病原体名称未给出]，1周后，第2组和第3组皮下注射达氟沙星。收集直肠粪便，通过培养确定FQ-R[病原体名称未给出]的相对百分比。在口服接种[病原体名称未给出]之前，87%的犊牛自然定植有FQ-R[病原体名称未给出]。接种后两天，大多数犊牛体内的FQ-R[病原体名称未给出]大幅下降。在注射达氟沙星的24小时内，两个FQ治疗组中的几乎所有[病原体名称未给出]群体都转变为FQ-R表型，但这只是暂时的，因为FQ-S菌株在后期成为优势菌株。基因分型表明，注射后FQ-R[病原体名称未给出]群体中出现的峰值主要是由于预先存在的FQ-R[病原体名称未给出]的富集，而不是易感菌株中从头产生FQ耐药性。这些结果为牛群中FQ耐药[病原体名称未给出]对FQ治疗的动态变化提供了重要见解。
