Skowronek Patrycja, Wójcik Łukasz, Strachecka Aneta
Department of Invertebrate Ecophysiology and Experimental Biology, University of Life Sciences in Lublin, Doświadczalna 50a, 20-280 Lublin, Poland.
Antioxidants (Basel). 2022 Apr 2;11(4):707. doi: 10.3390/antiox11040707.
We examined the effect of hemp extract on the activity of the antioxidant system (catalase, peroxidase, glutathione, superoxide dismutase, and total antioxidant capacity) in the hemolymph of adult honey bees (). The bees were divided into three groups: (1) an experimental group fed with pure sugar syrup with cotton strips soaked with hemp extract put inside the cage; (2) an experimental group fed with a mixture of sugar syrup with hemp extract; and (3) a control group fed with a mixture of sugar and a water-glycerine solution. Hemolymph samples were collected on the 1st day of this study and then every week, until all bees in the group died. The activities of all antioxidant enzymes were higher for the experimental groups, compared to those for the control group. The highest antioxidant activities were noted in the group supplemented with cannabis with the use of syringes. Supplementation with hemp also increased the lifespan of bees in this group compared to that of the bees consuming only sugar syrup (control: 35 days), with 49 and 52 days for groups of cannabis on strips and in syrup, respectively. Hemp extract, thanks to its antioxidant properties, increased the activities of key antioxidant enzymes that protect the bee's organisms against free radicals and thus delay the aging processes.
我们研究了大麻提取物对成年蜜蜂血淋巴中抗氧化系统（过氧化氢酶、过氧化物酶、谷胱甘肽、超氧化物歧化酶和总抗氧化能力）活性的影响。蜜蜂被分为三组：（1）实验组，在笼内放入浸泡过大麻提取物的棉条，喂食纯糖浆；（2）实验组，喂食添加了大麻提取物的糖浆混合物；（3）对照组，喂食糖和水 - 甘油溶液的混合物。在本研究的第1天采集血淋巴样本，之后每周采集一次，直至该组所有蜜蜂死亡。与对照组相比，实验组所有抗氧化酶的活性更高。在使用注射器添加大麻的组中，抗氧化活性最高。与仅食用糖浆的蜜蜂（对照组：35天）相比，添加大麻也延长了该组蜜蜂的寿命，添加在棉条上和糖浆中的大麻组蜜蜂寿命分别为49天和52天。大麻提取物因其抗氧化特性，提高了关键抗氧化酶的活性，这些酶可保护蜜蜂机体免受自由基侵害，从而延缓衰老过程。
