Jing Feng-Ya, Weng Yu-Jie, Zhang Yu-Qing
School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215123, China.
Antioxidants (Basel). 2022 Apr 5;11(4):712. doi: 10.3390/antiox11040712.
Two types of sericin peptides with high molecular weight (HS) and low molecular weight (LS) were obtained by the green water boiling ultrasonic method and the Ca(OH) ultrasonic method, respectively. In this experiment, a high-glucose medium was used to simulate a high-glucose environment in the body, and appropriate concentrations of normal alpha mouse liver 12 (AML12) hepatocytes were exposed to a series of concentrations of HS and LS. The effects of the two sericin peptides on AML12 cells in a high-glucose environment were investigated in detail in terms of oxidative stress and inflammatory factor expression in cells. HS and LS-groups reduced the levels of oxidative stress, inflammation, and tumor necrosis factor (TNF), and the latter significantly reduced the levels of TNF-α, interleukin (IL)-6, and nuclear factor (NF)-κB in AML12 cells. Additionally, it significantly reduced the oxidative stress damage caused by the high-glucose environment compared with normal AML12 cells. These results indicate that sericin may be an antioxidant recovered from industrial waste, and has potential and for use in the reduction of environmental pollution and the development of functional foods with antioxidation and antihyperglycemic effect.
分别通过绿水煮超声法和氢氧化钙超声法获得了两种高分子量（HS）和低分子量（丝胶蛋白肽）（LS）。在本实验中，使用高糖培养基模拟体内的高糖环境，并将适当浓度的正常α小鼠肝脏12（AML12）肝细胞暴露于一系列浓度的HS和LS中。从细胞中的氧化应激和炎症因子表达方面详细研究了两种丝胶蛋白肽在高糖环境中对AML12细胞的影响。HS组和LS组降低了氧化应激、炎症和肿瘤坏死因子（TNF）水平，后者显著降低了AML12细胞中TNF-α、白细胞介素（IL）-6和核因子（NF）-κB的水平。此外，与正常AML12细胞相比，它显著降低了高糖环境引起的氧化应激损伤。这些结果表明，丝胶蛋白可能是一种从工业废料中回收的抗氧化剂，在减少环境污染以及开发具有抗氧化和抗高血糖作用的功能性食品方面具有潜力。
