Rzymowska Jolanta, Wilkołaski Andrzej, Szatkowska Lidia, Grzybowska Ludmiła
Department of Biology and Genetics, Medical University of Lublin, Chodźki 4A, 20-093 Lublin, Poland.
Cardiology Clinic, Independent Public Clinical Hospital Nr 4, Chodźki 8, 20-093 Lublin, Poland.
Biology (Basel). 2022 Apr 3;11(4):555. doi: 10.3390/biology11040555.
The aim of the study was to investigate the effect of paclitaxel on the expression of genes encoding signaling factors in breast cancer cells in in vitro conditions after incubation with the said chemotherapeutic. The tested cells were harvested from the mammary glands of 36 patients with early breast cancer. The microarray technology was employed for the identification of gene expression. For this purpose, mRNA isolated from tumor cells was used. A significant effect of paclitaxel on the genome of breast cancer cells was confirmed. Paclitaxel changed the functions of cancer cells by increasing the expression of most genes encoding signaling proteins and receptors. The analysis of the results suggested that this cytostatic agent produces a beneficial therapeutic effect at a lower dose (60 ng/mL). In contrast, a high dose of paclitaxel (300 ng/mL) was associated with a high cytotoxicity.
本研究的目的是调查紫杉醇在体外条件下与所述化疗药物孵育后对乳腺癌细胞中编码信号因子的基因表达的影响。受试细胞取自36例早期乳腺癌患者的乳腺。采用微阵列技术鉴定基因表达。为此，使用了从肿瘤细胞中分离的mRNA。证实了紫杉醇对乳腺癌细胞基因组有显著影响。紫杉醇通过增加大多数编码信号蛋白和受体的基因的表达来改变癌细胞的功能。结果分析表明，这种细胞抑制剂在较低剂量（60 ng/mL）时产生有益的治疗效果。相比之下，高剂量的紫杉醇（300 ng/mL）具有高细胞毒性。
