Elnomrosy Sara M, Hagag Naglaa M, AbdAllah Mohamed I, Kolenda Rafał, Zacharski Maciej
Genome Research Unit, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Nadi El-Said Street, Dokki, Giza 12618, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Biology (Basel). 2022 Apr 8;11(4):565. doi: 10.3390/biology11040565.
Over 400 of the 3800 tropical avian species are endangered or threatened. One of many solutions to conserve animal biodiversity is breeding animals in zoos or private animal farms. Animal breeding programs are difficult to implement in species with sexual monomorphism, such as parrots. Molecular biology methods offer a solution to determine the sex of these species. Therefore, in this study, we aimed to test the performance of PCR and LAMP techniques on sex identification for 21 parrot species belonging to three families, i.e., , , and . We established a protocol for DNA isolation from feathers in our laboratory and found optimal conditions for PCR and LAMP. We showed that the LAMP method with the use of the PSI-W primers set, developed by Centeno-Cuadros, functions in 17 previously untested species. Moreover, we found that further improvements are required in universal LAMP primers for the detection of parrot DNA, which are necessary for confirmation of the male sex. The LAMP method also proved to be more sensitive for female sex identification in contrast to the reference PCR test. Therefore, we conclude that LAMP is a suitable method for the routine diagnostic sex identification of parrots.
3800种热带鸟类中有400多种濒临灭绝或受到威胁。保护动物生物多样性的众多解决方案之一是在动物园或私人养殖场繁殖动物。动物繁殖计划在具有性别单态性的物种（如鹦鹉）中难以实施。分子生物学方法为确定这些物种的性别提供了一种解决方案。因此，在本研究中，我们旨在测试聚合酶链式反应（PCR）和环介导等温扩增技术（LAMP）对三个科（即 、 和 ）的21种鹦鹉进行性别鉴定的性能。我们在实验室建立了从羽毛中提取DNA的方案，并找到了PCR和LAMP的最佳条件。我们表明，使用Centeno-Cuadros开发的PSI-W引物组的LAMP方法在17种以前未测试过的物种中有效。此外，我们发现用于检测鹦鹉DNA的通用LAMP引物需要进一步改进，这对于确认雄性性别是必要的。与参考PCR测试相比，LAMP方法在雌性性别鉴定中也被证明更灵敏。因此，我们得出结论，LAMP是鹦鹉常规诊断性别鉴定的合适方法。
