Sex Dev. 2019;13(1):47-54. doi: 10.1159/000495383. Epub 2018 Dec 21.
The use of accurate and reliable techniques for sex determination of wild birds is of special importance in captive breeding programs, especially in birds with monogamous, aggressive behavior, with absence of copulation, and with a low hatching rate. Using PCR, we evaluated the relative efficacy of primers HPF/HPR and CHD1Wr/NP/CHD1Zr in the amplification of the chromo-helicase-DNA binding 1 (CHD1) gene for sex determination in Penelope albipennis and 8 other species of cracids, 4 species of falconids, 4 species of accipitrids, and 3 species of psittacines. Primer effectiveness was compared using previously sexed bird samples. The HPF/HPR primer set was found to demonstrate a better performance and reliability. Therefore, these primers should be used to determine the sex of juvenile birds to avoid or minimize incompatibilities during the selection of potential breeding pairs.
使用准确可靠的技术来确定野生鸟类的性别在圈养繁殖计划中尤为重要，特别是对于那些具有单配制、攻击性强、缺乏交配行为和低孵化率的鸟类。本研究采用 PCR 技术，评估了 HPF/HPR 和 CHD1Wr/NP/CHD1Zr 这两组引物在扩增染色体解旋酶-DNA 结合蛋白 1（CHD1）基因以确定白喉林鸽和其他 8 种凤头鹃形目鸟类、4 种隼形目鸟类、4 种鹰形目鸟类和 3 种鹦鹉目鸟类性别方面的相对有效性。通过对已鉴定性别的鸟类样本进行比较，评估了引物的有效性。研究结果表明，HPF/HPR 引物组具有更好的性能和可靠性。因此，这些引物应被用于确定幼鸟的性别，以避免或最小化在选择潜在繁殖对时的不兼容性。
