Islam Md Shafiqul, Takagi Mitsuhiro, Lee Keun-Woo, Chang Hye-Sook, Okawa Hiroaki, Yunus Muchammad, Lestari Tita Damayanti, Tacharina Martia Rani, Pervin Shahnaj, Rakib Tofazzal Md, Yabuki Akira, Yamato Osamu
Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram 4225, Bangladesh.
Animals (Basel). 2022 Apr 17;12(8):1044. doi: 10.3390/ani12081044.
Immune adaptation plays an essential role in determining pregnancy, which has been shown to be dependent on sufficient immunological tolerance mediated by FOXP3+ regulatory T cells. Recently, an X-linked maternal single-nucleotide polymorphism (SNP), located 2175 base pairs upstream of the start codon in the bovine FOXP3 gene (NC_037357.1: g.87298881A>G, rs135720414), was identified in Japanese Black (JB: Bos taurus) cows in association with recurrent infertility. However, with the exception of JB cows, the frequency of this SNP has yet to be studied in other cow populations. In this study, we thus aimed to evaluate the frequency of this SNP in different cow breeds. Between 2018 and 2021, a total of 809 DNA samples were obtained from 581 JB, 73 Holstein Friesian (HF: B. taurus), 125 Korean Hanwoo (KH: B. taurus coreanae), and 30 Indonesian Madura (IM: a crossbreed between B. indicus and B. javanicus) cows, which were genotyped using a TaqMan probe-based real-time polymerase chain reaction assay designed in this study. The frequency of the G allele was found to be relatively high in local IM (0.700), moderate in dairy HF (0.466), and low in beef JB (0.250) and KH (0.112) cows, with differences in the frequencies between each group being shown to be statistically significant (p < 0.005) using Fisher’s exact test. The results obtained in this study indicate that the G allele frequencies of the identified the SNP differ markedly in different breeds of taurine and indicine cattle. Given these findings, it would thus be important to evaluate the relationships between high frequencies of the G allele and infertility in different breeds.
免疫适应在决定妊娠过程中起着至关重要的作用，研究表明这依赖于由FOXP3 + 调节性T细胞介导的足够的免疫耐受性。最近，在日本黑牛（JB：Bos taurus）中发现了一种X连锁的母体单核苷酸多态性（SNP），位于牛FOXP3基因起始密码子上游2175个碱基对处（NC_037357.1：g.87298881A>G，rs135720414），与反复性不孕有关。然而，除了JB牛之外，该SNP在其他牛群中的频率尚未得到研究。因此，在本研究中，我们旨在评估该SNP在不同牛品种中的频率。在2018年至2021年期间，总共从581头JB牛、73头荷斯坦弗里生牛（HF：Bos taurus）、125头韩国韩牛（KH：Bos taurus coreanae）和30头印度尼西亚马都拉牛（IM：印度瘤牛和爪哇牛的杂交品种）中获取了809份DNA样本，使用本研究设计的基于TaqMan探针的实时聚合酶链反应分析对其进行基因分型。发现G等位基因频率在本地IM牛中相对较高（0.700），在奶牛HF中中等（0.466），在肉牛JB（0.250）和KH（0.112）牛中较低，使用Fisher精确检验显示每组之间的频率差异具有统计学意义（p < 0.005）。本研究获得的结果表明，所鉴定的SNP的G等位基因频率在不同品种的普通牛和瘤牛中存在显著差异。鉴于这些发现，评估G等位基因高频率与不同品种不孕之间的关系将很重要。
