Faruq Abdullah Al, Widodo Oky Setyo, Takagi Mitsuhiro, Lestari Tita Damayanti, Mursalim Muhammad Fadhlullah, Laksono Nanang Tedjo, Okawa Hiroaki, Islam Md Shafiqul, Maki Shinichiro, Rakib Tofazzal Md, Yabuki Akira, Yamato Osamu
Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram 4225, Bangladesh.
Animals (Basel). 2025 Aug 16;15(16):2407. doi: 10.3390/ani15162407.
Reproductive failure in cattle production is a global concern and is influenced by various factors, including genetic alterations. This study explored the relationship between an X-linked single-nucleotide variant (NC_037357.1: g.87298881A>G, rs135720414) in the upstream of the bovine forkhead box P3 () gene and infertility. To this end, we examined the genotypes of the variant in old Asian cattle breeds, including 48 Bali and 5 Jaliteng cattle, and 20 water buffaloes, which have recently shown subclinical signs of infertility and repeated breeding problems among populations in Indonesia. We also examined the genotypes in 69 parous and 39 non-parous Holstein Friesian (HF) cows and investigated the relationship between the genotypes and serum concentration of anti-Müllerian hormone (AMH). The G allele frequency was markedly high in Bali (0.944) and Jaliteng cattle (0.714), and water buffaloes (1), suggesting that the G allele may be originally a wild-type variant in old Asian cattle and buffaloes. In HF cows, the G allele frequency was moderately high, and the AMH concentration was significantly lower ( < 0.05) in parous cows carrying the G allele (A/G and G/G genotypes) than in parous cows with the A/A genotype. In contrast, there were no significant differences in AMH concentrations among the three genotypes of non-parous HF cows. This suggests that both G allele and aging are associated with infertility in HF cows. In conclusion, the G allele of the gene variant may potentially be associated with infertility in different bovine breeds and species. Therefore, special attention should be paid to this variant, and infertility in bovine herds may be improved by selection and/or introduction of the A allele.
肉牛生产中的繁殖失败是一个全球性问题，受到包括基因改变在内的多种因素影响。本研究探讨了牛叉头框P3（）基因上游一个X连锁单核苷酸变异（NC_037357.1: g.87298881A>G，rs135720414）与不育之间的关系。为此，我们检测了古老亚洲牛品种中该变异的基因型，包括48头巴厘牛和5头加里滕牛，以及20头水牛，这些牛最近在印度尼西亚的种群中出现了亚临床不育迹象和反复配种问题。我们还检测了69头经产和39头未产荷斯坦弗里生（HF）奶牛的基因型，并研究了这些基因型与抗缪勒管激素（AMH）血清浓度之间的关系。巴厘牛（0.944）、加里滕牛（0.714）和水牛（1）中G等位基因频率显著较高，这表明G等位基因可能原本是古老亚洲牛和水牛中的野生型变异。在HF奶牛中，G等位基因频率适中偏高，携带G等位基因（A/G和G/G基因型）的经产奶牛的AMH浓度显著低于（<0.05）具有A/A基因型的经产奶牛。相比之下，未产HF奶牛的三种基因型之间的AMH浓度没有显著差异。这表明G等位基因和衰老都与HF奶牛的不育有关。总之，该基因变异的G等位基因可能与不同牛品种和物种的不育有关。因此，应特别关注该变异，通过选择和/或引入A等位基因可能改善牛群的不育情况。
