Elblehi Samar S, Abd El-Maksoud Eman M, Aldhahrani Adil, Alotaibi Saqer S, Ghamry Heba I, Elgendy Salwa A, Soliman Mohamed Mohamed, Shukry Mustafa
Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt.
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt.
Life (Basel). 2022 Apr 13;12(4):578. doi: 10.3390/life12040578.
This study aimed to investigate the oxidative neurotoxicity induced by silver nanoparticles (AgNPs) and assess the neuroprotective effects of quercetin against this toxicity. Forty adult male rats were divided into four equal groups: control, AgNPs (50 mg/kg intraperitoneally), quercetin (50 mg/kg orally), and quercetin + AgNPs. After 30 days, blood and brain tissue samples were collected for further studies. AgNP exposure increased lipid peroxidation and decreased glutathione peroxidase, catalase, and superoxide dismutase activities in brain tissue. AgNPs decreased serum acetylcholine esterase activity and γ-aminobutyric acid concentrations. AgNPs upregulated tumor necrosis factor-α, interleukin-1β, and transcript levels. AgNPs reduced the transcripts of claudin-5, brain-derived neurotrophic factor, paraoxonase, nuclear factor-erythroid factor 2 (Nrf2), and . Histopathologically, AgNPs caused various degenerative changes and neuronal necrosis associated with glial cell reactions. AgNPs increased the immunohistochemical staining of glial fibrillary acidic protein (GFAP) in the cerebrum and cerebellum. Oral treatment with quercetin efficiently counteracted the opposing effects of AgNPs on brain tissue via modulation of tight junction proteins, Nrf2, and paraoxonase, and its positive mechanism in modulating pro-inflammatory cytokines and the downregulation of GFAP expression, and the apoptotic pathway. AgNPs also altered the severity of histopathological lesions and modulated GFAP immunostaining in the examined tissue.
本研究旨在探讨银纳米颗粒（AgNPs）诱导的氧化神经毒性，并评估槲皮素对这种毒性的神经保护作用。将40只成年雄性大鼠分为四组，每组数量相等：对照组、AgNPs组（腹腔注射50 mg/kg）、槲皮素组（口服50 mg/kg）和槲皮素+AgNPs组。30天后，采集血液和脑组织样本进行进一步研究。暴露于AgNP会增加脑组织中的脂质过氧化，并降低谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶的活性。AgNPs会降低血清乙酰胆碱酯酶活性和γ-氨基丁酸浓度。AgNPs会上调肿瘤坏死因子-α、白细胞介素-1β和转录水平。AgNPs会降低紧密连接蛋白-5、脑源性神经营养因子、对氧磷酶、核因子-红细胞系2（Nrf2）和的转录本。组织病理学上，AgNPs会引起各种退行性变化和与胶质细胞反应相关的神经元坏死。AgNPs会增加大脑和小脑中胶质纤维酸性蛋白（GFAP）的免疫组化染色。口服槲皮素可通过调节紧密连接蛋白、Nrf2和对氧磷酶，以及其在调节促炎细胞因子和下调GFAP表达及凋亡途径方面的积极机制，有效对抗AgNPs对脑组织的相反作用。AgNPs还改变了所检查组织中组织病理学损伤的严重程度并调节了GFAP免疫染色。
