Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Food Funct. 2021 Jan 21;12(2):717-725. doi: 10.1039/d0fo01900c. Epub 2020 Dec 18.
Quercetin is one of the most abundant dietary flavonoid compounds, and its mechanism for combating age-related neurodegenerative diseases is unclear. In this study, quercetin (35 and 70 mg kg, orally administered for 4 weeks) was administered to 7-month-old aging mice (senescence-accelerated mouse prone 8 mice). As a result, it was found that quercetin could improve spatial learning and memory impairment displayed by aging mice in the Morris water maze. The results of immunoblotting reflected the protein expressions of the longevity factor (sirtuin1), inflammasomes (NLRP3 and ASC), synaptic marker (PSD95) and neurotrophic factors (BDNF and NGF) in the hippocampus of the brain. It indicated that the intervention of quercetin could increase the expression of sirtuin1 and prevent neuroinflammation, which was evident from the decrease in the protein levels of the astrocyte marker (GFAP) and inflammatory factors (cleaved-caspase 1, IL-1β and IL-18). In addition, quercetin could reduce the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) in the hippocampus of aging mice. Current data indicated that quercetin might improve neuroinflammation in aging mice by regulating the Sirtuin1/NLRP3 pathway.
槲皮素是最丰富的饮食类黄酮化合物之一，其对抗与年龄相关的神经退行性疾病的机制尚不清楚。在这项研究中，给 7 个月大的衰老小鼠（快速老化小鼠品系 8 型）口服给予槲皮素（35 和 70mg/kg，连续 4 周）。结果发现，槲皮素可以改善衰老小鼠在 Morris 水迷宫中表现出的空间学习和记忆障碍。免疫印迹的结果反映了大脑海马体中长寿因子（Sirtuin1）、炎性小体（NLRP3 和 ASC）、突触标志物（PSD95）和神经营养因子（BDNF 和 NGF）的蛋白表达。表明槲皮素的干预可以增加 Sirtuin1 的表达并预防神经炎症，这从星形胶质细胞标志物（GFAP）和炎症因子（cleaved-caspase 1、IL-1β 和 IL-18）的蛋白水平降低可以看出。此外，槲皮素可以降低衰老小鼠海马体中的丙二醛（MDA）和活性氧（ROS）水平。目前的数据表明，槲皮素可能通过调节 Sirtuin1/NLRP3 通路改善衰老小鼠的神经炎症。
