Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Animal Science, Shanxi Agricultural University, Taigu, China.
Anim Biotechnol. 2024 Nov;35(1):2254568. doi: 10.1080/10495398.2023.2254568. Epub 2023 Sep 11.
The thyroid gland is an important endocrine gland in animals, which mainly secretes thyroid hormones and acts on various organs of the body. Long-chain non-coding RNA (lncRNA) plays an important role in animal reproduction. However, there is still a lack of understanding of their expression patterns and potential roles in the thyroid of Small Tail Han (STH) sheep. In this study, RNA-seq was used to examine the transcriptome expression patterns of lncRNAs and mRNAs in the follicular phase (ww_FT) and luteal phase (ww_LT) in genotype STH Sheep. A total of 17,217 lncRNAs and 39,112 mRNAs were identified including 96 differentially expressed lncRNAs (DELs) and 1054 differentially expressed mRNAs (DEGs). Functional analysis of genes with significant differences in expression level showed that these genes could be enriched in Ras signalling pathway, hedgehog (HH) signalling pathway, ATP-binding cassette (ABC) transporters and other signalling pathways related to animal reproduction. In addition, through correlation analysis for lncRNA-mRNA co-expression and network construction, we found that LNC_009115 and LNC_005796 trans target NIK-related kinase () and poly(A)-specific ribonuclease (). LNC_007189 and LNC_002045 trans target progesterone-induced blocking factor 1 (), LNC_009013 trans targets small mothers against decapentaplegic () are related to animal reproduction. These genes add new resources for elucidating the regulatory mechanisms of reproduction in sheep with different reproductive cycles of the genotype STH sheep.
甲状腺是动物体内重要的内分泌腺，主要分泌甲状腺激素，作用于全身各器官。长链非编码 RNA（lncRNA）在动物繁殖中发挥重要作用。然而，lncRNA 在小尾寒羊甲状腺中的表达模式及其潜在作用仍知之甚少。本研究利用 RNA-seq 技术检测了基因型小尾寒羊卵泡期（ww_FT）和黄体期（ww_LT）甲状腺组织的 lncRNA 和 mRNA 转录组表达谱。共鉴定出 17217 个 lncRNA 和 39112 个 mRNA，包括 96 个差异表达的 lncRNA（DELs）和 1054 个差异表达的 mRNA（DEGs）。对表达水平有显著差异的基因进行功能分析表明，这些基因可富集于 Ras 信号通路、 hedgehog（HH）信号通路、ATP 结合盒（ABC）转运体等与动物繁殖相关的信号通路。此外，通过 lncRNA-mRNA 共表达的相关性分析和网络构建，发现 LNC_009115 和 LNC_005796 靶向 NIK 相关激酶（）和多聚（A）特异性核糖核酸酶（）。LNC_007189 和 LNC_002045 靶向孕激素诱导阻断因子 1（），LNC_009013 靶向 small mothers against decapentaplegic（），这些基因与动物繁殖有关。这些基因为阐明基因型小尾寒羊不同繁殖周期的繁殖调控机制提供了新的资源。
