Programa de Doctorado en Ciencias de la Salud, Instituto de Ciencias de la Salud, Universidad Veracruzana, Av. Luis, Dr. Castelazo Ayala s/n, Col. Industrial Animas, Xalapa 91190, Mexico.
Biomedical Institute of Valencia IBV-CSIC, C. de Jaume Roig, 11, 46010 Valencia, Spain.
Genes (Basel). 2022 Mar 29;13(4):609. doi: 10.3390/genes13040609.
Genes related to DNA damage repair in Mycobacterium tuberculosis are critical for survival and genomic diversification. The aim of this study is to compare the presence of SNPs in genes related to DNA damage repair in sensitive and drug-resistant M. tuberculosis genomes isolated from patients with and without type 2 diabetes mellitus (T2DM). We collected 399 M. tuberculosis L4 genomes from several public repositories; 224 genomes belonging to hosts without T2DM, of which 123 (54.9%) had drug sensitive tuberculosis (TB) and 101 (45.1%) had drug resistance (DR)-TB; and 175 genomes from individuals with T2DM, of which 100 (57.1%) had drug sensitive TB and 75 (42.9%) had DR-TB. The presence of SNPs in the coding regions of 65 genes related to DNA damage repair was analyzed and compared with the resistance profile and the presence/absence of T2DM in the host. The results show the phylogenetic relationships of some SNPS and L4 sub-lineages, as well as differences in the distribution of SNPs present in DNA damage repair-related genes related to the resistance profile of the infecting strain and the presence of T2DM in the host. Given these differences, it was possible to generate two discriminant functions to distinguish between drug sensitive and drug resistant genomes, as well as patients with or without T2DM.
结核分枝杆菌中与 DNA 损伤修复相关的基因对于生存和基因组多样化至关重要。本研究旨在比较来自患有 2 型糖尿病（T2DM）和不患有 T2DM 的患者的敏感和耐药结核分枝杆菌基因组中与 DNA 损伤修复相关的基因中 SNP 的存在情况。我们从多个公共存储库中收集了 399 株结核分枝杆菌 L4 基因组；224 个基因组属于不患有 T2DM 的宿主，其中 123 个（54.9%）具有药物敏感结核病（TB），101 个（45.1%）具有耐药性（DR）-TB；175 个基因组来自患有 T2DM 的个体，其中 100 个（57.1%）具有药物敏感 TB，75 个（42.9%）具有 DR-TB。分析了与 DNA 损伤修复相关的 65 个基因的编码区中 SNP 的存在情况，并与宿主中的耐药谱和 T2DM 的存在情况进行了比较。结果表明了一些 SNP 和 L4 亚谱系的系统发育关系，以及与感染株的耐药谱和宿主中 T2DM 的存在相关的 DNA 损伤修复相关基因中存在的 SNP 的分布差异。鉴于这些差异，可以生成两个判别函数来区分敏感和耐药基因组，以及患有或不患有 T2DM 的患者。
