雄激素调控与前列腺腺泡腺癌中 microRNAs 的相关性研究。
Association of Androgenic Regulation and MicroRNAs in Acinar Adenocarcinoma of Prostate.
机构信息
Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém 66050-160, Brazil.
Núcleo de Pesquisas em Oncologia, Universidade Federal do Pará, Belém 66073-005, Brazil.
出版信息
Genes (Basel). 2022 Mar 30;13(4):622. doi: 10.3390/genes13040622.
BACKGROUND
Prostate cancer represents 3.8% of cancer deaths worldwide. For most prostate cancer cells to grow, androgens need to bind to a cellular protein called the androgen receptor (AR). This study aims to demonstrate the expression of five microRNAs (miRs) and its influence on the AR formation in patients from the northern region of Brazil.
MATERIAL AND METHODS
Eighty-four tissue samples were investigated, including nodular prostatic hyperplasia (NPH) and acinar prostatic adenocarcinoma (CaP). Five miRs (27a-3p, 124, 130a, 488-3p, and 506) were quantified using the TaqMan Real Time PCR method and AR was measured using Western blotting.
RESULTS
Levels of miRs 124, 130a, 488-3p, and 506 were higher in NPH samples. Conversely, in the CaP cases, higher levels of miR 27a-3p and AR were observed.
CONCLUSION
In the future, these microRNAs may be tested as markers of CaP at the serum level. The relative expression of AR was 20% higher in patients with prostate cancer, which suggests its potential as a biomarker for prostate malignancy.
背景
前列腺癌占全球癌症死亡人数的 3.8%。大多数前列腺癌细胞的生长需要雄激素与一种叫做雄激素受体（AR）的细胞蛋白结合。本研究旨在展示巴西北部地区患者中五种 microRNAs（miRs）的表达及其对 AR 形成的影响。
材料和方法
研究了 84 个组织样本，包括结节性前列腺增生（NPH）和腺癌（CaP）。使用 TaqMan 实时 PCR 方法定量检测了五种 miRs（27a-3p、124、130a、488-3p 和 506），并使用 Western blot 检测 AR。
结果
NPH 样本中 miR 124、130a、488-3p 和 506 的水平较高。相反，在 CaP 病例中，miR 27a-3p 和 AR 的水平较高。
结论
未来，这些 microRNAs 可能会在血清水平上作为 CaP 的标志物进行测试。前列腺癌患者的 AR 相对表达水平高出 20%，这表明其可能成为前列腺恶性肿瘤的生物标志物。
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