Singh Amandeep, Yemmireddy Veerachandra
School of Earth, Environmental and Marine Sciences, University of Texas Rio Grande Valley, 1201 W University Dr, Edinburg, TX 78539, USA.
Department of Biology, University of Texas Rio Grande Valley, 1201 W University Dr, Edinburg, TX 78539, USA.
Microorganisms. 2022 Apr 8;10(4):786. doi: 10.3390/microorganisms10040786.
Foodborne pathogens such as , O157:H7, and are known to survive under different environmental stresses with an effect on their physiological properties. The purpose of this study was to determine the effect of different environmental stresses on the foodborne pathogens response to subsequent chemical treatments. Three types of pathogens , O157:H7, and were subjected to different environmental stresses: (i) Desiccation (ii) high salt (iii) low pH, and (iv) temperatures (14, 23, and 37 °C) during their growth. The cells harvested at their early stationary growth phase were subsequently subjected to chlorine (100 or 200 ppm), peracetic acid (40 or 80 ppm), and 0.5% lactic acid treatments. The results showed that pre-growth stress conditions have significant effect on the reduction of tested pathogens depending upon the type of chemical treatment. showed the highest sensitivity against all these treatments when compared to O157:H7 and . In addition, showed the highest percentage of sub-lethally injured cells. These findings highlighted the need to consider pre-growth conditions as an important factor for the validation of physical and chemical intervention treatments.
诸如大肠杆菌O157:H7等食源性病原体已知可在不同环境压力下存活，并对其生理特性产生影响。本研究的目的是确定不同环境压力对食源性病原体对后续化学处理反应的影响。三种类型的病原体，即大肠杆菌O157:H7和单核细胞增生李斯特菌，在其生长过程中受到不同的环境压力：（i）干燥（ii）高盐（iii）低pH值，以及（iv）温度（14、23和37°C）。在其生长早期稳定期收获的细胞随后接受氯（100或200 ppm）、过氧乙酸（40或80 ppm）和0.5%乳酸处理。结果表明，预生长应激条件对受试病原体的减少有显著影响，这取决于化学处理的类型。与大肠杆菌O157:H7和单核细胞增生李斯特菌相比，鼠伤寒沙门氏菌对所有这些处理表现出最高的敏感性。此外，鼠伤寒沙门氏菌显示出亚致死损伤细胞的百分比最高。这些发现突出了将预生长条件视为物理和化学干预处理验证的重要因素的必要性。
