Institute of Cereal Crop, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2022 Apr 18;23(8):4446. doi: 10.3390/ijms23084446.
Leaf size is an important agronomic trait directly affecting yield in rice, and thus understanding the genes determining leaf size is important in breeding. In this study, one () with small leaf size was isolated using ethyl methane sulphonate (EMS) mutagenesis from the Zhenggeng 1925. MutMap by whole genome resequencing of phenotypic bulks revealed that is likely located in the 133 kb region on chromosome 7 using F population from a cross between and wild-type (WT) Zhenggeng 1925. The candidate gene encoding heat shock protein for was functionally validated. Disruption of this gene in mutants significantly reduced the leaf size compared with that of WT in rice. Microscopic examination showed that modulated leaf size via regulating the veins formation and cell size/cell number. Nucleotide diversity analysis indicated that a single nucleotide polymorphism (SNP) variation of C to T in the coding region of may cause small leaves among rice accessions. Therefore, the natural variation of contributing to leaf size might be useful for rice breeding.
叶片大小是一个重要的农艺性状，直接影响水稻的产量，因此了解决定叶片大小的基因在育种中很重要。本研究利用甲基磺酸乙酯（EMS）诱变从粳稻品种珍桂 1925 中分离到一个叶片小的突变体 ()。利用表型群体的全基因组重测序进行 MutMap 分析，发现 可能位于 7 号染色体上 133 kb 的区域，该群体是由 与野生型（WT）珍桂 1925 杂交的 F2 群体构建的。候选基因编码热休克蛋白 ，该基因可能是 。与 WT 相比， 突变体中该基因的破坏显著降低了叶片大小。显微镜检查表明， 通过调节叶脉形成和细胞大小/细胞数量来调节叶片大小。核苷酸多样性分析表明， 编码区 C 到 T 的单核苷酸多态性（SNP）变异可能导致水稻品种的叶片变小。因此， 对叶片大小的自然变异可能对水稻育种有用。
